SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUBX5
Systematic nameYDR330W
DescriptionUBX domain-containing protein 5

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06682
SGD YDR330W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
139Phosphoserine.

Sequence Information
Sequence length500
Molecular weight56737

	---------+----------+----------+----------+----------+
MSEGKVDEFM AITGADDAAI ATQFIEMADG NLNTAISLFF ENGGAALLSS   50
NNTPTPSNST PMAPTSVDSD ADAQLAERLQ REAYQQQQPD QDYVRPPDEA   100
RHEVLTETSG FPISYGGIGG RFEPLHRVND MFDEGRPESI FNQRLDDTNT   150
NTYINDNSSD SLDSEEENDD DEYEYVEEPV IELDEDGNIK EYTKLVRKPK   200
TISKEQKLAL LFRPPFSIMS KLDLDAAKQK ARAKQKWIMI NIQDSGIFQC   250
QALNRDLWSS RPVKTIIKEN FVFLQYQYES RNAQPYLQFY HLNNKDDLPH   300
IAILDPITGE RVKQWNRVVP IPEQFISEIN EFLASFSLDP KVPNPTVNEP   350
LPKVDPTTLT EEQQMELAIK ESLNNNSSKS NQEEVPSTGE EQKRVQEPDP   400
FSTIEARVHP EPPNKPGITT RIQIRTGDGS RLVRRFNALE DTVRTIYEVI   450
KTEMDGFADS RFTLNDHQRE DLIDKLNMTI ADAGLKNSSL LLEKLDPEIE   500