SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameUBR2
Systematic nameYLR024C
DescriptionE3 ubiquitin-protein ligase UBR2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K709        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]

Cross References
Swiss-Prot accessionQ07963
SGD YLR024C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
EC number 6.3.2.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
712Phosphoserine.
1222Phosphoserine.

Sequence Information
Sequence length1872
Molecular weight216778

	---------+----------+----------+----------+----------+
MEDSDLSITN IRDFLTELPK LAKCEYSETT SYLLWKTLNL RLKHSDNDIN   50
WRSLVSILNS EAWENEKYRD ILNGRKWRTL EFENDHHSVG NMHIGTACTR   100
LCFPSETIYY CFTCSTNPLY EICELCFDKE KHVNHSYVAK VVMRPEGRIC   150
HCGDPFAFND PSDAFKCKNE LNNIPISNDN SNVTDDENVI SLLNYVLDFL   200
IDVTVSYKEE AEAHSSERKA SSLMHPNQNS ITDDIMEKHE CEPLVNDENF   250
VFFDNNWSNT RKEAHMEWAI QIEEEECNVH YMDLASTITR ILNTPVEYAI   300
SITKALEDSH DVVTVLQSEN FFEIDQIAKE FQKENIVVHV RKADDIFKRK   350
LTDDLTDWLY SLCFKAATSL QNKYALRISM LDVWYSHFSK MRVSPTNTNP   400
DFSKINLLGG FLISNEDSDE SWFKPWSLEN IEDERISKIL TNYNERLIRA   450
HSPNTVSHFY NFYGSRFQYI IINSINILSK KSKFKMLKIM ASLFSLRDES   500
RKFLAAQYID VYLSVLYDAV ASDAKECQVT LMSILGQYTF QDPSIANMTI   550
SSGFIERTIR FAFTLMAFNP EDLMSYLPIS LYNGFKLPTE TIRNRRTIIC   600
FKDLCTIMSA NTVPEELLSN EAIFNAIIES FSEFSNVLPL KRETKEHVEV   650
ENFDFSAFYF FFSSILIMTD GYTRSISLVK DAAFRKQIVL KLLDVAQTRE   700
FESLTNSRKA ISPDNASTNE NDSNKATLST VRETICNYVA ETINFQVGVN   750
TQYFFNPMSY LFKFVIQWSQ CGRYEPIPAS LTNYINLFEV FQDKQKALYI   800
SESALSTLVL IGQINVGFWV RNGTPITHQA RMYTKYSMRE FTYISDIFNV   850
QFSMAMCNPD ELMVTYLSRW GLKHWANGVP MYDYPDTETT VAVVNECILL   900
LIQLLTEVRS LVMKSSKEGF ERTFKSEIIH ALCFDTCSYA QIVNCIPEHI   950
TKHPSFDIYL EKYANYTSPV SLTDNGIFVL KEKYKDEIDP YYIGLSSSRR   1000
YDVEKNIRLN MANLKKMKYE DTFVPAKKVK DLLKNTLFSG LYSISSVNTF   1050
GLFLKNTLDH IIKYDYDNLL PRVVHLIHLC VVNNLNEFMG ILWHEYAIVD   1100
TEFCHYHSIG SILYYCLLKD NFSESHGKIR EIFRYLMETA PHVNVNSYLR   1150
EQTTSYTPGI LWPTKEDKSH KDKEFERKKH LARLRKKKLM KKLAQQQMKF   1200
MENNSVDTSD ISTPRTTSPS LSPTRINAEN SSNTINSCCD DDCVFCKMPK   1250
DDDVFVYFSY QERNICDHGI DFTNPTDVNR INSLFSGKQT KDSAIQENPQ   1300
DDDGTRLKFT SCEPVLRACG HGSHTKCLSG HMKSIRGIQN QTTKNIPLSY   1350
GSGLIYCPVC NSLSNSFLPK TNDIDKRTSS QFFMCIEKRS EAEENLDPMS   1400
SICIKAAMIL GDLQGKKVTT IEDAYKVVNS VFINTISNTE LRLRSHKKEG   1450
KIVNMERISS QCILTLHLVC ELKSFIYKKF VNSKTFSSEI SRKIWNWNEF   1500
LIKGNNVNLL LYMSQNFDNI DGGKTPQPPN LCIYEMFKRR FHQLLLLLAR   1550
DMMRVNFYKD CRNKIKISSN GSEEPSTSFS YLFNTFKKYV DLFKPDDVRF   1600
DFTSLEKIKD FICSLLLESL SIFCRRTFLL FNIQYDDDGD GDNNNNRSNN   1650
FMDVKQREIE LIFRYFKLPN LTHFLKDFFY NELTQNIERY NDGNDNLRIQ   1700
QVIYDMVQNI NTRAYPSPEH IQLIELPLNL SKFSLDNDEI SNKCDKYEIA   1750
VCLLCGQKCH IQKSIALQGY LQGECTDHMR NGCEITSAYG VFLMTGTNAI   1800
YLSYGKRGTF YAAPYLSKYG ETNEDYKFGT PVYLNRARYA NLANEIVFGN   1850
MIPHIVFRLT DGSADLGGWE TM                                 1872

	* Ubiquitinated lysine is denoted as red color.