SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUBC8
Systematic nameYEL012W
AliasGID3
DescriptionUbiquitin-conjugating enzyme E2-24 kDa

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP28263
SGD YEL012W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0045721negative regulation of gluconeogenesisIMP:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
Biological processGO:0042787protein ubiquitination during ubiquitin-dep...IMP:SGD.
EC number 6.3.2.19

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length218
Molecular weight24633

	---------+----------+----------+----------+----------+
MSSSKRRIET DVMKLLMSDH QVDLINDSMQ EFHVKFLGPK DTPYENGVWR   50
LHVELPDNYP YKSPSIGFVN KIFHPNIDIA SGSICLDVIN STWSPLYDLI   100
NIVEWMIPGL LKEPNGSDPL NNEAATLQLR DKKLYEEKIK EYIDKYATKE   150
KYQQMFGGDN DSDDSDSGGD LQEEDSDSDE DMDGTGVSSG DDSVDELSED   200
LSDIDVSDDD DYDEVANQ                                      218