SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUBC6
Systematic nameYER100W
AliasDOA2
DescriptionUbiquitin-conjugating enzyme E2 6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTIUBC7     Proteasomal Degradation
2 MULTI       
3   RINGSPRFSSM4   
4         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Sec61p-independent degradation of the tail-anchored ER membrane protein Ubc6p."
Walter J, Urban J, Volkwein C, Sommer T.
EMBO J. 20(12):3124-31 (2001) [11406589]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[3]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways."
Ravid T, Kreft SG, Hochstrasser M.
EMBO J. 25(3):533-43 (2006) [16437165]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP33296
SGD YER100W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0030433ER-associated protein catabolic processIGI:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
EC number 6.3.2.19

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
178Phosphothreonine.

Sequence Information
Sequence length250
Molecular weight28383

	---------+----------+----------+----------+----------+
MATKQAHKRL TKEYKLMVEN PPPYILARPN EDNILEWHYI ITGPADTPYK   50
GGQYHGTLTF PSDYPYKPPA IRMITPNGRF KPNTRLCLSM SDYHPDTWNP   100
GWSVSTILNG LLSFMTSDEA TTGSITTSDH QKKTLARNSI SYNTFQNVRF   150
KLIFPEVVQE NVETLEKRKL DEGDAANTGD ETEDPFTKAA KEKVISLEEI   200
LDPEDRIRAE QALRQSENNS KKDGKEPNDS SSMVYIGIAI FLFLVGLFMK   250