SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameUBC4
Systematic nameYBR082C
DescriptionUbiquitin-conjugating enzyme E2 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP15731
SGD YBR082C
Gene Ontology (GO)
Cellular componentGO:0000502proteasome complex (sensu Eukaryota)IPI:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0006513protein monoubiquitinationIDA:SGD.
Biological processGO:0000209protein polyubiquitinationIDA:SGD.
Biological processGO:0006950response to stressIDA:SGD.
Biological processGO:0030437sporulation (sensu Fungi)TAS:SGD.
Biological processGO:0043162ubiquitin-dependent protein catabolic proce...IMP:SGD.
EC number 6.3.2.19

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length148
Molecular weight16456

	---------+----------+----------+----------+----------+
MSSSKRIAKE LSDLERDPPT SCSAGPVGDD LYHWQASIMG PADSPYAGGV   50
FFLSIHFPTD YPFKPPKISF TTKIYHPNIN ANGNICLDIL KDQWSPALTL   100
SKVLLSICSL LTDANPDDPL VPEIAHIYKT DRPKYEATAR EWTKKYAV     148