SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTYW1
Systematic nameYPL207W
DescriptiontRNA wybutosine-synthesizing protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K496        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08960
SGD YPL207W
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Biological processGO:0031591wybutosine biosynthetic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length810
Molecular weight89805

	---------+----------+----------+----------+----------+
MDGFRVAGAL VVGALTAAYL YFGGRFSIAL VIIVGYGIYC NEASGGSQDS   50
QEKLDLNKQQ KKPCCSDKKI ADGGKKTGGC CSDKKNGGGK GGGCCSSKGG   100
KKGGCCSSKG GKKGGCCSSK KNIGDNENTA TEVEKAVNYP VTVDFTEVFR   150
KPTKKRSSTP KVFSKNSSSN SRVGKKLSVS KKIGPDGLIK SALTISNETL   200
LSSQIYVLYS SLQGAASKAA KSVYDKLKEL DELTNEPKLL NLDDLSDFDD   250
YFINVPVENA LYVLVLPSYD IDCPLDYFLQ TLEENANDFR VDSFPLRKLV   300
GYTVLGLGDS ESWPEKFCYQ AKRADHWISR LGGRRIFPLG KVCMKTGGSA   350
KIDEWTSLLA ETLKDDEPII YEYDENADSE EDEEEGNGSD ELGDVEDIGG   400
KGSNGKFSGA DEIKQMVAKD SPTYKNLTKQ GYKVIGSHSG VKICRWTKNE   450
LRGKGSCYKK SLFNIASSRC MELTPSLACS SKCVFCWRHG TNPVSKNWRW   500
EVDEPEYILE NALKGHYSMI KQMRGVPGVI AERFAKAFEV RHCALSLVGE   550
PILYPHINKF IQLLHQKGIT SFLVCNAQHP EALRNIVKVT QLYVSIDAPT   600
KTELKKVDRP LYKDFWERMV ECLEILKTVQ NHQRTVFRLT LVKGFNMGDV   650
SAYADLVQRG LPGFIEVKGA TFSGSSDGNG NPLTMQNIPF YEECVKFVKA   700
FTTELQRRGL HYDLAAEHAH SNCLLIADTK FKINGEWHTH IDFDKFFVLL   750
NSGKDFTYMD YLEKTPEWAL FGNGGFAPGN TRVYRKDKKK QNKENQETTT   800
RETPLPPIPA                                                810

	* Ubiquitinated lysine is denoted as red color.