SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTYS1
Systematic nameYGR185C
AliasMGM104
DescriptionTyrosyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP36421
SGD YGR185C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004831tyrosine-tRNA ligase activityIDA:SGD.
Biological processGO:0006437tyrosyl-tRNA aminoacylationIDA:SGD.
EC number 6.1.1.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.

Sequence Information
Sequence length394
Molecular weight44020

	---------+----------+----------+----------+----------+
MSSAATVDPN EAFGLITKNL QEVLNPQIIK DVLEVQKRHL KLYWGTAPTG   50
RPHCGYFVPM TKLADFLKAG CEVTVLLADL HAFLDNMKAP LEVVNYRAKY   100
YELTIKAILR SINVPIEKLK FVVGSSYQLT PDYTMDIFRL SNIVSQNDAK   150
RAGADVVKQV ANPLLSGLIY PLMQALDEQF LDVDCQFGGV DQRKIFVLAE   200
ENLPSLGYKK RAHLMNPMVP GLAQGGKMSA SDPNSKIDLL EEPKQVKKKI   250
NSAFCSPGNV EENGLLSFVQ YVIAPIQELK FGTNHFEFFI DRPEKFGGPI   300
TYKSFEEMKL AFKEEKLSPP DLKIGVADAI NELLEPIRQE FANNKEFQEA   350
SEKGYPVATP QKSKKAKKPK NKGTKYPGAT KTNEIATKLE ETKL         394