SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTY1B-JR1
Systematic nameYJR027W
AliasYJRWTy1-1 POL
DescriptionReverse transcriptase/ribonuclease H

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP47098
SGD YJR027W
Gene Ontology (GO)
Molecular functionGO:0004523ribonuclease H activityIEA:EC.
EC number 3.4.23.-,2.7.7.49,2.7.7.7,3.1.26.4

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1N-acetylmethionine.
13Phosphoserine.
47Phosphoserine.

Sequence Information
Sequence length1755
Molecular weight198616

	---------+----------+----------+----------+----------+
MESQQLSQHS HISHGSACAS VTSKEVHTNQ DPLDVSASKT EECEKASTKA   50
NSQQTTTPAS SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG   100
WSFYGHPSMI PYTPYQMSPM YFPPGPQSQF PQYPSSVGTP LSTPSPESGN   150
TFTDSSSADS DMTSTKKYVR PPPMLTSPND FPNWVKTYIK FLQNSNLGGI   200
IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI LSVDYTDIMK   250
ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN   300
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ   350
QGSRNSKPNY RRNLSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA   400
HNVSTSNNSP STDNDSISKS TTEPIQLNNK HDLTLGQELT ESTVNHTNHS   450
DDELPGHLLL DSGASRTLIR SAHHIHSASS NPDINVVDAQ KRNIPINAIG   500
DLQFHFQDNT KTSIKVLHTP NIAYDLLSLN ELAAVDITAC FTKNVLERSD   550
GTVLAPIVQY GDFYWVSKRY LLPSNISVPT INNVHTSEST RKYPYPFIHR   600
MLAHANAQTI RYSLKNNTIT YFNESDVDWS SAIDYQCPDC LIGKSTKHRH   650
IKGSRLKYQN SYEPFQYLHT DIFGPVHNLP KSAPSYFISF TDETTKFRWV   700
YPLHDRREDS ILDVFTTILA FIKNQFQASV LVIQMDRGSE YTNRTLHKFL   750
EKNGITPCYT TTADSRAHGV AERLNRTLLD DCRTQLQCSG LPNHLWFSAI   800
EFSTIVRNSL ASPKSKKSAR QHAGLAGLDI STLLPFGQPV IVNDHNPNSK   850
IHPRGIPGYA LHPSRNSYGY IIYLPSLKKT VDTTNYVILQ GKESRLDQFN   900
YDALTFDEDL NRLTASYQSF IASNEIQESN DLNIESDHDF QSDIELHPEQ   950
PRNVLSKAVS PTDSTPPSTH TEDSKRVSKT NIRAPREVDP NISESNILPS   1000
KKRSSTPQIS NIESTGSGGM HKLNVPLLAP MSQSNTHESS HASKSKDFRH   1050
SDSYSENETN HTNVPISSTG GTNNKTVPQI SDQETEKRII HRSPSIDASP   1100
PENNSSHNIV PIKTPTTVSE QNTEESIIAD LPLPDLPPES PTEFPDPFKE   1150
LPPINSHQTN SSLGGIGDSN AYTTINSKKR SLEDNETEIK VSRDTWNTKN   1200
MRSLEPPRSK KRIHLIAAVK AVKSIKPIRT TLRYDEAITY NKDIKEKEKY   1250
IEAYHKEVNQ LLKMKTWDTD EYYDRKEIDP KRVINSMFIF NKKRDGTHKA   1300
RFVARGDIQH PDTYDTGMQS NTVHHYALMT SLSLALDNNY YITQLDISSA   1350
YLYADIKEEL YIRPPPHLGM NDKLIRLKKS HYGLKQSGAN WYETIKSYLI   1400
KQCGMEEVRG WSCVFKNSQV TICLFVDDMI LFSKDLNANK KIITTLKKQY   1450
DTKIINLGES DNEIQYDILG LEIKYQRGKY MKLGMENSLT EKIPKLNVPL   1500
NPKGRKLSAP GQPGLYIDQD ELEIDEDEYK EKVHEMQKLI GLASYVGYKF   1550
RFDLLYYINT LAQHILFPSR QVLDMTYELI QFMWDTRDKQ LIWHKNKPTE   1600
PDNKLVAISD ASYGNQPYYK SQIGNIFLLN GKVIGGKSTK ASLTCTSTTE   1650
AEIHAISESV PLLNNLSYLI QELNKKPIIK GLLTDSRSTI SIIKSTNEEK   1700
FRNRFFGTKA MRLRDEVSGN NLYVYYIETK KNIADVMTKP LPIKTFKLLT   1750
NKWIH                                                    1755