SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTUB1
Systematic nameYML085C
DescriptionTubulin alpha-1 chain

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP09733
SGD YML085C
Gene Ontology (GO)
Cellular componentGO:0005881cytoplasmic microtubuleTAS:SGD.
Cellular componentGO:0005828kinetochore microtubuleTAS:SGD.
Cellular componentGO:0005880nuclear microtubuleTAS:SGD.
Cellular componentGO:0005827polar microtubuleTAS:SGD.
Cellular componentGO:0005816spindle pole bodyIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0005200structural constituent of cytoskeletonTAS:SGD.
Biological processGO:0045143homologous chromosome segregationTAS:SGD.
Biological processGO:0000070mitotic sister chromatid segregationTAS:SGD.
Biological processGO:0000743nuclear migration during conjugation with c...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
274Phosphoserine.

Sequence Information
Sequence length447
Molecular weight49800

	---------+----------+----------+----------+----------+
MREVISINVG QAGCQIGNAC WELYSLEHGI KPDGHLEDGL SKPKGGEEGF   50
STFFHETGYG KFVPRAIYVD LEPNVIDEVR NGPYKDLFHP EQLISGKEDA   100
ANNYARGHYT VGREILGDVL DRIRKLADQC DGLQGFLFTH SLGGGTGSGL   150
GSLLLEELSA EYGKKSKLEF AVYPAPQVST SVVEPYNTVL TTHTTLEHAD   200
CTFMVDNEAI YDMCKRNLDI PRPSFANLNN LIAQVVSSVT ASLRFDGSLN   250
VDLNEFQTNL VPYPRIHFPL VSYSPVLSKS KAFHESNSVS EITNACFEPG   300
NQMVKCDPRD GKYMATCLLY RGDVVTRDVQ RAVEQVKNKK TVQLVDWCPT   350
GFKIGICYEP PTATPNSQLA TVDRAVCMLS NTTSIAEAWK RIDRKFDLMY   400
AKRAFVHWYV GEGMEEGEFT EAREDLAALE RDYIEVGADS YAEEEEF      447