SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTSL1
Systematic nameYML100W
DescriptionTrehalose synthase complex regulatory subunit TSL1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38427
SGD YML100W
Gene Ontology (GO)
Cellular componentGO:0005946alpha,alpha-trehalose-phosphate synthase co...IGI:SGD.
Molecular functionGO:0030234enzyme regulator activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006950response to stressIMP:SGD.
Biological processGO:0005992trehalose biosynthetic processIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
49Phosphoserine.
75Phosphothreonine.
77Phosphoserine.
79Phosphoserine.
147Phosphoserine.
161Phosphoserine.
192Phosphoserine.
247Phosphothreonine.
251Phosphothreonine.

Sequence Information
Sequence length1098
Molecular weight123018

	---------+----------+----------+----------+----------+
MALIVASLFL PYQPQFELDT SLPENSQVDS SLVNIQAMAN DQQQQRALSN   50
NISQESLVAP APEQGVPPAI SRSATRSPSA FNRASSTTNT ATLDDLVSSD   100
IFMENLTANA TTSHTPTSKT MLKPRKNGSV ERFFSPSSNI PTDRIASPIQ   150
HEHDSGSRIA SPIQQQQQDP TTNLLKNVNK SLLVHSLLNN TSQTSLEGPN   200
NHIVTPKSRA GNRPTSAATS LVNRTKQGSA SSGSSGSSAP PSIKRITPHL   250
TASAAKQRPL LAKQPSNLKY SELADISSSE TSSQHNESDP DDLTTAPDEE   300
YVSDLEMDDA KQDYKVPKFG GYSNKSKLKK YALLRSSQEL FSRLPWSIVP   350
SIKGNGAMKN AINTAVLENI IPHRHVKWVG TVGIPTDEIP ENILANISDS   400
LKDKYDSYPV LTDDDTFKAA YKNYCKQILW PTLHYQIPDN PNSKAFEDHS   450
WKFYRNLNQR FADAIVKIYK KGDTIWIHDY HLMLVPQMVR DVLPFAKIGF   500
TLHVSFPSSE VFRCLAQREK ILEGLTGADF VGFQTREYAR HFLQTSNRLL   550
MADVVHDEEL KYNGRVVSVR FTPVGIDAFD LQSQLKDGSV MQWRQLIRER   600
WQGKKLIVCR DQFDRIRGIH KKLLAYEKFL VENPEYVEKS TLIQICIGSS   650
KDVELERQIM IVVDRINSLS TNISISQPVV FLHQDLDFSQ YLALSSEADL   700
FVVSSLREGM NLTCHEFIVC SEDKNAPLLL SEFTGSASLL NDGAIIINPW   750
DTKNFSQAIL KGLEMPFDKR RPQWKKLMKD IINNDSTNWI KTSLQDIHIS   800
WQFNQEGSKI FKLNTKTLME DYQSSKKRMF VFNIAEPPSS RMISILNDMT   850
SKGNIVYIMN SFPKPILENL YSRVQNIGLI AENGAYVSLN GVWYNIVDQV   900
DWRNDVAKIL EDKVERLPGS YYKINESMIK FHTENAEDQD RVASVIGDAI   950
THINTVFDHR GIHAYVYKNV VSVQQVGLSL SAAQFLFRFY NSASDPLDTS   1000
SGQITNIQTP SQQNPSDQEQ QPPASPTVSM NHIDFACVSG SSSPVLEPLF   1050
KLVNDEASEG QVKAGHAIVY GDATSTYAKE HVNGLNELFT IISRIIED     1098