SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTRM1
Systematic nameYDR120C
DescriptionN(2),N(2)-dimethylguanosine tRNA methyltransferase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP15565
SGD YDR120C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionTAS:SGD.
Cellular componentGO:0005637nuclear inner membraneTAS:SGD.
Molecular functionGO:0004809tRNA (guanine-N2-)-methyltransferase activityIDA:SGD.
Biological processGO:0030488tRNA methylationIMP:SGD.
EC number 2.1.1.32

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length570
Molecular weight64052

	---------+----------+----------+----------+----------+
MEGFFRIPLK RANLHGMLKA AISKIKANFT AYGAPRINIE DFNIVKEGKA   50
EILFPKKETV FYNPIQQFNR DLSVTCIKAW DNLYGEECGQ KRNNKKSKKK   100
RCAETNDDSS KRQKMGNGSP KEAVGNSNRN EPYINILEAL SATGLRAIRY   150
AHEIPHVREV IANDLLPEAV ESIKRNVEYN SVENIVKPNL DDANVLMYRN   200
KATNNKFHVI DLDPYGTVTP FVDAAIQSIE EGGLMLVTCT DLSVLAGNGY   250
PEKCFALYGG ANMVSHESTH ESALRLVLNL LKQTAAKYKK TVEPLLSLSI   300
DFYVRVFVKV KTSPIEVKNV MSSTMTTYHC SRCGSYHNQP LGRISQREGR   350
NNKTFTKYSV AQGPPVDTKC KFCEGTYHLA GPMYAGPLHN KEFIEEVLRI   400
NKEEHRDQDD TYGTRKRIEG MLSLAKNELS DSPFYFSPNH IASVIKLQVP   450
PLKKVVAGLG SLGFECSLTH AQPSSLKTNA PWDAIWYVMQ KCDDEKKDLS   500
KMNPNTTGYK ILSAMPGWLS GTVKSEYDSK LSFAPNEQSG NIEKLRKLKI   550
VRYQENPTKN WGPKARPNTS                                     570