SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTRE1
Systematic nameYPL176C
DescriptionUncharacterized protein TRE1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5  Vacuolar Degradation
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Multiple interactions drive adaptor-mediated recruitment of the ubiquitin ligase rsp5 to membrane proteins in vivo and in vitro."
Sullivan JA, Lewis MJ, Nikko E, Pelham HR.
Mol Biol Cell. 18(7):2429-40 (2007) [17429078]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionQ08919
SGD YPL176C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
597Phosphoserine.

Sequence Information
Sequence length783
Molecular weight88756

	---------+----------+----------+----------+----------+
MVNTRGYTTL PNVEEPANNS QDELNSQDFE QAIGMPSEPP VYVEEMGMEE   50
PQAPEAFSEK VQRFRMCFEN NVVIPVKKNV VDPLAQMISL ASEKFDLFLS   100
KIGNVMVMRR IFYIMMMSII AALIIASDRL PNGKARGSNG SFSDHDLLLQ   150
YARKSIDLSK IERDLEYISS MPHMSGTSGD AAIRHYIKES FDKNGIRLAG   200
EEEFMAYSNY PGNVSLRVYS KDDTEGFDIP LNEENFNPMS HNGQLNNIPV   250
IYANKASLDD MASMQDQGLL NGDFILLVHY GDYVFQQMLT AQEYGAKAII   300
FISEPYQDNK DVIQMKSVAL PQYGTGDALT PEWEGSIRDP IDATEAKCLP   350
KIPSIPISAN QGDKILAILS DTGVKFSNNL FSGSLNDCRL DLLVQTAIRE   400
RHPVHDIVGK IEGSEQAGRA IVIAAPRNSA SYGTMYPSFG TVVLLSLIQL   450
YQEMVYKFDW KPLRNIYFIS FGGSEFNEAG ATELMEKRTE ALKSEIYTII   500
DVGQIGIWDD SNNLEIQCHP LLVDLFQKNM TSRKFNVKVD NVHQFGDWTP   550
YLAQGIPVAI ISSPGVMNRE HPIYTVEDKF DFIKDKLRDK KKGEVLSEIM   600
LYLVEKSLEL IDDPFIPFSI SNYVDFLSTT LKDLQKECPD TVNFDEVFLG   650
TTLWENTKLQ FEKWKSEWTE LMYGAGTYIE PTIIAINRWS WNYLLSLIGV   700
TQCLEEGLMD RTFYKNVIFG PKLWVDKGDP LRSWTFPEIR DTIAIKDWSS   750
VQVQANTLGT ILQNTARYFL ENKNLHGINT NEF                     783