SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTPS2
Systematic nameYDR074W
AliasPFK3
DescriptionTrehalose-phosphatase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP31688
SGD YDR074W
Gene Ontology (GO)
Cellular componentGO:0005946alpha,alpha-trehalose-phosphate synthase co...IGI:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004805trehalose-phosphatase activityIMP:SGD.
Biological processGO:0006950response to stressIDA:SGD.
Biological processGO:0005992trehalose biosynthetic processIMP:SGD.
EC number 3.1.3.12

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
890Phosphoserine.

Sequence Information
Sequence length896
Molecular weight102976

	---------+----------+----------+----------+----------+
MTTTAQDNSP KKRQRIINCV TQLPYKIQLG ESNDDWKISA TTGNSALFSS   50
LEYLQFDSTE YEQHVVGWTG EITRTERNLF TREAKEKPQD LDDDPLYLTK   100
EQINGLTTTL QDHMKSDKEA KTDTTQTAPV TNNVHPVWLL RKNQSRWRNY   150
AEKVIWPTFH YILNPSNEGE QEKNWWYDYV KFNEAYAQKI GEVYRKGDII   200
WIHDYYLLLL PQLLRMKFND ESIIIGYFHH APWPSNEYFR CLPRRKQILD   250
GLVGANRICF QNESFSRHFV SSCKRLLDAT AKKSKNSSNS DQYQVSVYGG   300
DVLVDSLPIG VNTTQILKDA FTKDIDSKVL SIKQAYQNKK IIIGRDRLDS   350
VRGVVQKLRA FETFLAMYPE WRDQVVLIQV SSPTANRNSP QTIRLEQQVN   400
ELVNSINSEY GNLNFSPVQH YYMRIPKDVY LSLLRVADLC LITSVRDGMN   450
TTALEYVTVK SHMSNFLCYG NPLILSEFSG SSNVLKDAIV VNPWDSVAVA   500
KSINMALKLD KEEKSNLESK LWKEVPTIQD WTNKFLSSLK EQASSNDDME   550
RKMTPALNRP VLLENYKQAK RRLFLFDYDG TLTPIVKDPA AAIPSARLYT   600
ILQKLCADPH NQIWIISGRD QKFLNKWLGG KLPQLGLSAE HGCFMKDVSC   650
QDWVNLTEKV DMSWQVRVNE VMEEFTTRTP GSFIERKKVA LTWHYRRTVP   700
ELGEFHAKEL KEKLLSFTDD FDLEVMDGKA NIEVRPRFVN KGEIVKRLVW   750
HQHGKPQDML KGISEKLPKD EMPDFVLCLG DDFTDEDMFR QLNTIETCWK   800
EKYPDQKNQW GNYGFYPVTV GSASKKTVAK AHLTDPQQVL ETLGLLVGDV   850
SLFQSAGTVD LDSRGHVKNS ESSLKSKLAS KAYVMKRSAS YTGAKV       896