SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTPO3
Systematic nameYPR156C
DescriptionPolyamine transporter 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ06451
SGD YPR156C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Cellular componentGO:0005774vacuolar membraneIMP:SGD.
Molecular functionGO:0000297spermine transporter activityIMP:SGD.
Biological processGO:0015846polyamine transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
55Phosphoserine.
101Phosphoserine.
132Phosphoserine.

Sequence Information
Sequence length622
Molecular weight68066

	---------+----------+----------+----------+----------+
MNRQESINSF NSDETSSLSD VESQQPQQYI PSESGSKSNM APNQLKLTRT   50
ETVKSLQDMG VSSKAPVPDV NAPQSSKNKI FPEEYTLETP TGLVPVATLH   100
SIGRTSTAIS RTRTRQIDGA SSPSSNEDAL ESDNNEKGKE GDSSGANDEA   150
PDLDPEIEFV TFVTGDPENP HNWPAWIRWS YTVLLSILVI CVAYGSACIS   200
GGLGTVEKKY HVGMEAAILS VSLMVIGFSL GPLIWSPVSD LYGRRVAYFV   250
SMGLYVIFNI PCALAPNLGS LLACRFLCGV WSSSGLCLVG GSIADMFPSE   300
TRGKAIAFFA FAPYVGPVVG PLVNGFISVS TGRMDLIFWV NMAFAGVMWI   350
ISSAIPETYA PVILKRKAAR LRKETGNPKI MTEQEAQGVS MGEMMRACLL   400
RPLYFSVTEP VLVATCFYVC LIYSLLYAFF FAFPVIFGEL YGYKDNLVGL   450
MFIPIVIGAL WALATTFYCE NKYLQIVKQR KPTPEDRLLG AKIGAPFAAI   500
ALWILGATAY KHIIWVGPAS AGLAFGFGMV LIYYSLNNYI IDCYVQYASS   550
ALATKVFLRS AGGAAFPLFT IQMYHKLNLH WGSWLLAFIS TAMIALPFAF   600
SYWGKGLRHK LSKKDYSIDS IE                                 622