SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTPO2
Systematic nameYGR138C
DescriptionPolyamine transporter 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53283
SGD YGR138C
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Cellular componentGO:0005774vacuolar membraneIMP:SGD.
Molecular functionGO:0000297spermine transporter activityIMP:SGD.
Biological processGO:0015846polyamine transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
45Phosphothreonine.
47Phosphothreonine.
50Phosphoserine.
102Phosphoserine.
105Phosphoserine.

Sequence Information
Sequence length614
Molecular weight67589

	---------+----------+----------+----------+----------+
MSDQESVVSF NSQNTSMVDV EGQQPQQYVP SKTNSRANQL KLTKTETVKS   50
LQDLGVTSAA PVPDINAPQT AKNNIFPEEY TMETPSGLVP VATLQSMGRT   100
ASALSRTRTK QLNRTATNSS STGKEEMEEE ETEEREDQSG ENELDPEIEF   150
VTFVTGDPEN PHNWPSWVRW SYTVLLSILV ICVAYGSACI SGGLGTVEKK   200
YHVGMEAAIL SCSLMVIGFS LGPLIWSPVS DLYGRRVAYF VSMGLYVIFN   250
IPCALAPNLG CLLACRFLCG VWSSSGLCLV GGSIADMFPS ETRGKAIAFF   300
AFAPYVGPVV GPLVNGFISV STGRMDLIFW VNMAFAGVMW IISSAIPETY   350
APVILKRKAA RLRKETGNPK IMTEQEAQGV SMSEMMRACL LRPLYFAVTE   400
PVLVATCFYV CLIYSLLYAF FFAFPVIFGE LYGYKDNLVG LMFIPIVIGA   450
LWALATTFYC ENKYLQIVKQ RKPTPEDRLL GAKIGAPFAA IALWILGATA   500
YKHIIWVGPA SAGLAFGFGM VLIYYSLNNY IIDCYVQYAS SALATKVFLR   550
SAGGAAFPLF TIQMYHKLNL HWGSWLLAFI STAMIALPFA FSYWGKGLRH   600
KLSKKDYSID SVEM                                          614