SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTPO1
Systematic nameYLL028W
DescriptionPolyamine transporter 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ07824
SGD YLL028W
Gene Ontology (GO)
Cellular componentGO:0005933cellular budIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Cellular componentGO:0005774vacuolar membraneIMP:SGD.
Molecular functionGO:0015606spermidine transporter activityIMP:SGD.
Molecular functionGO:0000297spermine transporter activityIDA:SGD.
Biological processGO:0015846polyamine transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
19Phosphoserine, by PKC.
52Phosphothreonine, by CK1.
72Phosphoserine.
76Phosphoserine.
89Phosphothreonine.
91Phosphoserine.
342Phosphoserine, by PKA.

Sequence Information
Sequence length586
Molecular weight64272

	---------+----------+----------+----------+----------+
MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY   50
TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV   100
DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL   150
VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV   200
IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA   250
APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW   300
RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA   350
HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY   400
PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV   450
PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG   500
IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW   550
AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA                  586