SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTPI1
Systematic nameYDR050C
DescriptionTriosephosphate isomerase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP00942
SGD YDR050C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004807triose-phosphate isomerase activityIMP:SGD.
Biological processGO:0006096glycolysisIMP:SGD.
EC number 5.3.1.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
96Phosphoserine.
100Phosphoserine.

Sequence Information
Sequence length248
Molecular weight26795

	---------+----------+----------+----------+----------+
MARTFFVGGN FKLNGSKQSI KEIVERLNTA SIPENVEVVI CPPATYLDYS   50
VSLVKKPQVT VGAQNAYLKA SGAFTGENSV DQIKDVGAKW VILGHSERRS   100
YFHEDDKFIA DKTKFALGQG VGVILCIGET LEEKKAGKTL DVVERQLNAV   150
LEEVKDWTNV VVAYEPVWAI GTGLAATPED AQDIHASIRK FLASKLGDKA   200
ASELRILYGG SANGSNAVTF KDKADVDGFL VGGASLKPEF VDIINSRN     248