SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTOM1
Systematic nameYDR457W
AliasSSR2
DescriptionE3 ubiquitin-protein ligase TOM1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ03280
SGD YDR457W
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityTAS:SGD.
Biological processGO:0007067mitosisIMP:SGD.
Biological processGO:0006997nuclear organization and biogenesisIMP:SGD.
Biological processGO:0006913nucleocytoplasmic transportIMP:SGD.
Biological processGO:0006513protein monoubiquitinationTAS:SGD.
Biological processGO:0000209protein polyubiquitinationTAS:SGD.
Biological processGO:0008361regulation of cell sizeIMP:SGD.
EC number 6.3.2.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1890Phosphoserine.
2119Phosphoserine.
2185Phosphoserine.
2418Phosphoserine.

Sequence Information
Sequence length3268
Molecular weight374185

	---------+----------+----------+----------+----------+
MVLFTRCEKA RKEKLAAGYK PLVDYLIDCD TPTFLERIEA IQEWDRSRDD   50
LYVWIPILDR MDGLLLKVAE KYKYKQDPKK ECEVKLVEME AHDVDYCLKM   100
LKFTRRLLLN TENRFVYSSG DVLMYLLNCP NFTIKLAVMR ILAILGERFV   150
IAREKIVAHN IFGDHNLRKK TLKLALSLSS SVMDEDGEHF SLVDLYFDKK   200
KVPQKWRKLR FTHYTSNDFK KSSQQKNNIN ETQTSIKKVT MTTQELCEHS   250
LQQIFDKGMA LLPAESWFDF SIKASVAKAF SDDSGENIDL RNIIIETKLN   300
AIAFVNTIFS PPQVSSKLFE LDPYAFNSLT DLISLSETKI PKELRTDALF   350
TLECISLKHV WCSDIIRNLG GNISHGLLFQ ILRYIAKTLR EATDEIDEEY   400
NVRFFYLISN LADVKPLHES LFAAGLIPTL LEIVSIRNCP YKRTLASATH   450
LLETFIDNSE TTTEFIENDG FTMLITSVAN EIDFTLAHPE TWQPPKYSVV   500
YYSISFRELA YIRSLLKLVL KLLSTDSGDR IRNLIDSPIL VSLKKILENK   550
LVFGLTLITY TLDVVQKVIN SEPTIYPVLV EAGLIPYVID NFPKLIGPSA   600
ELLSLLPDVV SAICLNPEGL KQVKEKGLIN NLFDFLLDAD HARILTGGDR   650
STEYGTDIDE LARHYPDLKA NIVEALCNVI RKMPSTFRNE REFLFTSPKD   700
QKYFFHRKNE EILTDKEEHE PAYWELLDKG TMLDTFTSVL FGMSLGNGSF   750
SQVPQHLEAR DFLAIIFMEN PPYEYFTSVA ISNVTEVLQY LDEKYEDYAF   800
MDVMKVLNDQ LENLNDFLNS PNDRSFFLER DGENSVRSCH SKLCRLAAIL   850
NIVTNVYIDL TTLSCKRIMQ IYSYFDKRGF SLIKNLKLLF QKCALEEMYI   900
RQHMPDSVIT ETMPLPIVDV SGDGPPLQIY IDDPKKGDQK GKITSVKTRN   950
TLQMRTILYT LQSNTAILFR CFLRLSHSRN MDLEHKDLTT EVHIFENVVE   1000
NVIEMLKATE LEGHLPYILV LLNFNTFVFT IPKASPNSTE ILQTIPAYIF   1050
YQKGGYLLYL HIIRDLFTRM TKIKDLSSLD NINYIDESNG ILTLSCLINA   1100
LTFYNKSMQT ETMENVQSIG KYYVSIDDDY NIMKALTVPI KVMALAMILD   1150
LDKSDSLFKT QSRNVPYSVF KQLLSMLKNI FTNVNIYTKE LYELHWDLIF   1200
PPIKKISLFE QVGIPGDVAA NYLTDTGDDL PADNSIGLFS PEQWEKYKKL   1250
IGEDKSIYYP QPMQAQYYKG CSSKELDELR DTFFNDGLPS RIFTVLPFYP   1300
KLVNAFAKTL LQIFTKYDEP TEVFAGRILD RILETDLDDP ATLSSLIHLF   1350
GIFLNEKYIY QKASHLMQRF IEYLEKSLKP EHVNTPWFSK ALYVYEIILA   1400
KSELPHLEEL SKDVLLRYPL LSMAKVFRIP DPMKQKLFDI LIRVSDISNF   1450
YSALATSRIL IFYSRDELYA NNIARSGILS RLLKVIGSFQ KLDKINFLES   1500
SFLLLTRRCF ETTENVDALI RAEINRSFTA RPLGGGDDAV RELTTILEEK   1550
AHVVMRSPSQ FIDVLCETAR FHEFDDQGAL VDYSLKRFLG EKDKNTQASS   1600
TEKSDIYERT GIMHLLLSQL MAASEKDWLS EPANSSDLPE NKKAQLDPSR   1650
NPVCAYMIFL LKLLVELVSS YNQCKFEFLT FSRRNTYAER PRPRTTAINF   1700
FLYRLLDKPV GTDHDKHEAK RREVIGMLAR SVIIGFLATV QDDRTTKTDV   1750
KLADPHMNFI RKFAIEAIIK AIRNATSSSK LLESNHLKLD MWFRIITSMV   1800
YVQAPYLRQL LDSNKVEADQ YQLCKLVIDL GLPSVITEAM ASIDLNYPFS   1850
KKIFNVAVEA LNTISSTRNN FSEHFKIEDH DEVEDEVDES DKEEIPDMFK   1900
NSALGMYDVE DIEEDDDDDT SLIGDDDAMA FVDSDNGFEV VFSDEDDDMG   1950
EEDADDARSD SEENELSSEM QSSTADGTDV DYEVDDADGL IINIDQPSGD   2000
DEEMADYDAN ISHSSHSENE DDASMDVIEV YDDELSSGYD VDLSDYDVDE   2050
SDWDSGLSSL SISDEDSESS EDEPINSTRM GDSRRRWLIA EGVELTDDSQ   2100
GESEEDDRGV FRGIEHIFSN ENEPLFRVHD EMRHRNHHRS INRTHFHSAM   2150
SAPSLSLLNR GRRNQSNLIN PLGPTGLEQV ENDISDQVTV AGSGSRPRSH   2200
HLHFSEVLVS GSFFDEPVLD GIILKSTVSR WKDIFDMFYD SKTYANCIIP   2250
TVINRLYKVS LALQKDLENK REQEKLKNKN LLFNEAKVES HNSSDAISVE   2300
QDDIQESNVT HDDHEPVYVT IQGSEVDIGG TDIDPEFMNA LPDDIRADVF   2350
AQHVRERRAE ARLNSDHNVH SREIDSDFLE AIPEDIREGI LDTEAEEQRM   2400
FGRIGSSADV IRADDDVSNN DEEVENGLDH GNSNDRNNAD PEKKKPARIY   2450
FAPLIDRAGI ASLMKSVFIS KPYIQREIYH ELFYRLCSSK QNRNDLMNTF   2500
LFILSEGIID QHSLEKVYNI ISSRAMGHAK TTTVRQLPSD CTPLTVANQT   2550
IEILQSLIDA DSRLKYFLIA EHDNLIVNKA NNKSRKEALP DKKLRWPLWH   2600
LFSLLDRKLI TDESVLMDLL TRILQVCTKT LAVLSTSSNG KENLSKKFHL   2650
PSFDEDDLMK ILSIIMLDSC TTRVFQQTLN IIYNLSKLQG CMSIFTKHLV   2700
SLAISIMSKL KSALDGLSRE VGTITTGMEI NSELLQKFTL PSSDQAKLLK   2750
ILTTVDFLYT HKRKEEERNV KDLQSLYDKM NGGPVWSSLS ECLSQFEKSQ   2800
AINTSATILL PLIESLMVVC RRSDLSQNRN TAVKYEDAKL LDFSKTRVEN   2850
LFFPFTDAHK KLLNQMIRSN PKLMSGPFAL LVKNPKVLDF DNKRYFFNAK   2900
LKSDNQERPK LPITVRREQV FLDSYRALFF KTNDEIKNSK LEITFKGESG   2950
VDAGGVTREW YQVLSRQMFN PDYALFLPVP SDKTTFHPNR TSGINPEHLS   3000
FFKFIGMIIG KAIRDQCFLD CHFSREVYKN ILGRPVSLKD MESLDPDYYK   3050
SLVWILENDI TDIIEETFSV ETDDYGEHKV INLIEGGKDI IVTEANKQDY   3100
VKKVVEYKLQ TSVKEQMDNF LVGFYALISK DLITIFDEQE LELLISGLPD   3150
IDVDDWKNNT TYVNYTATCK EVSYFWRAVR SFDAEERAKL LQFVTGTSKV   3200
PLNGFKELSG VNGVCKFSIH RDFGSSERLP SSHTCFNQLN LPPYESYETL   3250
RGSLLLAINE GHEGFGLA                                      3268