SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTIP20
Systematic nameYGL145W
AliasTIP1
DescriptionProtein transport protein TIP20

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP33891
SGD YGL145W
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0006890retrograde vesicle-mediated transport, Golg...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length701
Molecular weight81167

	---------+----------+----------+----------+----------+
MNGIDDLLNI NDRIKQVQNE RNELASKLQN LKQSLASNDT EVALSEVIAQ   50
DIIEVGASVE GLEQLRAKYG DLQILNKLEK VAVQQTQMQA GVDKLDSFER   100
QLDELAEQPP DQFTLDDVKA LHSKLTSVFA TVPQINNIDS QYAAYNKLKS   150
KVTGKYNDVI IQRLATNWSN TFDQKLLEAQ WDTQKFASTS VGLVKCLREN   200
STKLYQLSLL YLPLEEETQN GDSERPLSRS NNNQEPVLWN FKSLANNFNV   250
RFTYHFHATS SSSKIETYFQ FLNDYLAENL YKCINIFHDD CNGLTKPVIH   300
EQFINYVLQP IRDKVRSTLF QNDLKTLIVL ISQILATDKN LLNSFHYHGL   350
GLVSLISDEV WEKWINYEVE MANRQFINIT KNPEDFPKSS QNFVKLINKI   400
YDYLEPFYDL DFDLLVRYKL MTCSLIFMNL TSSYLDYILT VDSLNETRTK   450
EQELYQTMAK LQHVNFVYRK IKSLSSNFIF IQLTDIVNST ESKKYNSLFQ   500
NVENDYEKAM STDMQNSIVH RIQKLLKETL RNYFKISTWS TLEMSVDENI   550
GPSSVPSAEL VNSINVLRRL INKLDSMDIP LAISLKVKNE LLNVIVNYFT   600
ESILKLNKFN QNGLNQFLHD FKSLSSILSL PSHATNYKCM SLHELVKILK   650
LKYDPNNQQF LNPEYIKTGN FTSLKEAYSI KYLKDTKIQD ALYRIIYGNI   700
L                                                        701