SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTIF34
Systematic nameYMR146C
DescriptionEukaryotic translation initiation factor 3 39 kDa subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40217
SGD YMR146C
Gene Ontology (GO)
Cellular componentGO:0005852eukaryotic translation initiation factor 3 ...IDA:SGD.
Cellular componentGO:0043614multi-eIF complexIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003743translation initiation factor activityIDA:SGD.
Biological processGO:0006413translational initiationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length347
Molecular weight38755

	---------+----------+----------+----------+----------+
MKAIKLTGHE RPLTQVKYNK EGDLLFSCSK DSSASVWYSL NGERLGTLDG   50
HTGTIWSIDV DCFTKYCVTG SADYSIKLWD VSNGQCVATW KSPVPVKRVE   100
FSPCGNYFLA ILDNVMKNPG SINIYEIERD SATHELTKVS EEPIHKIITH   150
EGLDAATVAG WSTKGKYIIA GHKDGKISKY DVSNNYEYVD SIDLHEKSIS   200
DMQFSPDLTY FITSSRDTNS FLVDVSTLQV LKKYETDCPL NTAVITPLKE   250
FIILGGGQEA KDVTTTSANE GKFEARFYHK IFEEEIGRVQ GHFGPLNTVA   300
ISPQGTSYAS GGEDGFIRLH HFEKSYFDFK YDVEKAAEAK EHMQEAN      347