SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTIF2
Systematic nameYJL138C
AliasTIF41B
DescriptionATP-dependent RNA helicase eIF4A

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP10081
SGD YJL138C
Gene Ontology (GO)
Cellular componentGO:0016281eukaryotic translation initiation factor 4F...IGI:SGD.
Cellular componentGO:0005840ribosomeTAS:SGD.
Molecular functionGO:0004004ATP-dependent RNA helicase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003743translation initiation factor activityIDA:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006413translational initiationIDA:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
2Phosphoserine.
146Phosphothreonine.

Sequence Information
Sequence length395
Molecular weight44697

	---------+----------+----------+----------+----------+
MSEGITDIEE SQIQTNYDKV VYKFDDMELD ENLLRGVFGY GFEEPSAIQQ   50
RAIMPIIEGH DVLAQAQSGT GKTGTFSIAA LQRIDTSVKA PQALMLAPTR   100
ELALQIQKVV MALAFHMDIK VHACIGGTSF VEDAEGLRDA QIVVGTPGRV   150
FDNIQRRRFR TDKIKMFILD EADEMLSSGF KEQIYQIFTL LPPTTQVVLL   200
SATMPNDVLE VTTKFMRNPV RILVKKDELT LEGIKQFYVN VEEEEYKYEC   250
LTDLYDSISV TQAVIFCNTR RKVEELTTKL RNDKFTVSAI YSDLPQQERD   300
TIMKEFRSGS SRILISTDLL ARGIDVQQVS LVINYDLPAN KENYIHRIGR   350
GGRFGRKGVA INFVTNEDVG AMRELEKFYS TQIEELPSDI ATLLN        395