SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTHS1
Systematic nameYIL078W
DescriptionThreonyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP04801
SGD YIL078W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006412translationIMP:SGD.
EC number 6.1.1.3

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length734
Molecular weight84520

	---------+----------+----------+----------+----------+
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE   50
ERIEMFDRLQ KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG   100
ISKSLADRLC ISKVNGQLWD LDRPFEGEAN EEIKLELLDF ESDEGKKVFW   150
HSSAHVLGES CECHLGAHIC LGPPTDDGFF YEMAVRDSMK DISESPERTV   200
SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK YKTYLVQTKV   250
PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS   300
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC   350
FWLPHGTRIY NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK   400
ENMFTFEVEK ETFGLKPMNC PGHCLMFKSR ERSYRELPWR VADFGVIHRN   450
EFSGALSGLT RVRRFQQDDA HIFCTHDQIE SEIENIFNFL QYIYGVFGFE   500
FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN AGDGAFYGPK   550
IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR   600
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL   650
HDAGFYADVD LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD   700
VMEQQGKNAT VSVEEVLKQL RNLKDEKRGD NVLA                    734