SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTHR1
Systematic nameYHR025W
DescriptionHomoserine kinase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP17423
SGD YHR025W
Gene Ontology (GO)
Molecular functionGO:0004413homoserine kinase activityIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0009092homoserine metabolic processIDA:SGD.
Biological processGO:0006549isoleucine metabolic processTAS:SGD.
Biological processGO:0006555methionine metabolic processTAS:SGD.
Biological processGO:0006566threonine metabolic processTAS:SGD.
EC number 2.7.1.39

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length357
Molecular weight38712

	---------+----------+----------+----------+----------+
MVRAFKIKVP ASSANIGPGY DVLGVGLSLF LELDVTIDSS QAQETNDDPN   50
NCKLSYTKES EGYSTVPLRS DANLITRTAL YVLRCNNIRN FPSGTKVHVS   100
NPIPLGRGLG SSGAAVVAGV ILGNEVAQLG FSKQRMLDYC LMIERHPDNI   150
TAAMMGGFCG SFLRDLTPQE VERREIPLAE VLPEPSGGED TGLVPPLPPT   200
DIGRHVKYQW NPAIKCIAII PQFELSTADS RGVLPKAYPT QDLVFNLQRL   250
AVLTTALTMD PPNADLIYPA MQDRVHQPYR KTLIPGLTEI LSCVTPSTYP   300
GLLGICLSGA GPTILALATE NFEEISQEII NRFAKNGIKC SWKLLEPAYD   350
GASVEQQ                                                  357