SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTHI22
Systematic nameYPR121W
DescriptionThiamine biosynthesis protein THI22

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06490
SGD YPR121W
Gene Ontology (GO)
Biological processGO:0009228thiamin biosynthetic processIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length572
Molecular weight63307

	---------+----------+----------+----------+----------+
MVIILLGLCT LGFPRTAFCP SIMTNSTVSI NTPPPYLTLA CNEKLPTVMS   50
IAGSDSSGGA GVEADIKTIT AHRCYAMTCV TTLTAQTPVK VYGAHNIPKK   100
MVSQILDANL QDMKCNVIKT GMLTVDAIEV LHEKLLQLGE NRPKLVIDPV   150
LCAASDSSPT GKDVVSLIIE KISPFADILT PNISDCFMLL GENREVSKLQ   200
DVLEIAKDLS RITNCSNILV KGGHIPCDDG KEKHITDVLY LGAEQKFITF   250
KGQFVNTTRT HGAGCTLASA IASNLARGYS LSQSVYGGIE YVQNAIAIGC   300
DVTKKAVKVG PINHVYAVEI PLEKMLTDEC FTASDAVPKK PIEGSLDKIP   350
GGSFFNYLIN HPKVKPHWDA YVNHEFVKRV ADGTLERKKF QFFIEQDYLY   400
LIDYVRVCCV TGSKSPTLED LEKDLVIADC ARNELNEHER RLREEFGVKD   450
PDYLQKIKRG PALRAYCRYL IDISRRGNWQ EIVVALNPCL MGYVYAVDKV   500
KDKITAAEGS IYSEWCDTCA SSFCYQAVLE GERLMNHILE TYPPDQLDSL   550
VTIFARGCEL ETNFWTAAME YE                                 572