SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTEF4
Systematic nameYKL081W
AliasEFC1
DescriptionElongation factor 1-gamma 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP36008
SGD YKL081W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005840ribosomeTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003746translation elongation factor activityTAS:SGD.
Biological processGO:0006414translational elongationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length412
Molecular weight46520

	---------+----------+----------+----------+----------+
MSQGTLYINR SPRNYASEAL ISYFKLDVKI VDLEQSSEFA SLFPLKQAPA   50
FLGPKGLKLT EALAIQFYLA NQVADEKERA RLLGSDVIEK SQILRWASLA   100
NSDVMSNIAR PFLSFKGLIP YNKKDVDACF VKIDNLAAVF DARLRDYTFV   150
ATENISLGDL HAAGSWAFGL ATILGPEWRA KHPHLMRWFN TVAASPIVKT   200
PFAEVKLAEK ALTYTPPKKQ KAEKPKAEKS KAEKKKDEAK PADDAAPAKK   250
PKHPLEALGK STFVLDDWKR KYSNDDTRPV ALPWFWEHYN PEEYSIWKVG   300
YKYNDELTLT FMSNNLVGGF FNRLSASTKY MFGCLVVYGE NNNNGIVGAV   350
MVRGQDFAPA FDVAPDWESY EYTKLDPTKE EDKEFVNNMW AWDKPVVVNG   400
EDKEIVDGKV LK                                            412