SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameTEF2
Systematic nameYBR118W
DescriptionElongation factor 1-alpha

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP02994
SGD YBR118W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIPI:SGD.
Cellular componentGO:0005840ribosomeTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003746translation elongation factor activityTAS:SGD.
Biological processGO:0006414translational elongationTAS:SGD.
Biological processGO:0006409tRNA export from nucleusIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1Blocked amino end (Met).
6Phosphoserine.
18Phosphoserine.
30N6-methyllysine.
72Phosphothreonine.
79N6,N6,N6-trimethyllysine.
163Phosphoserine.
316N6,N6-dimethyllysine.
390N6-methyllysine.
394Phosphoserine.
414Phosphoserine.
430Phosphothreonine.
458Lysine methyl ester.

Sequence Information
Sequence length458
Molecular weight50033

	---------+----------+----------+----------+----------+
MGKEKSHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAELG   50
KGSFKYAWVL DKLKAERERG ITIDIALWKF ETPKYQVTVI DAPGHRDFIK   100
NMITGTSQAD CAILIIAGGV GEFEAGISKD GQTREHALLA FTLGVRQLIV   150
AVNKMDSVKW DESRFQEIVK ETSNFIKKVG YNPKTVPFVP ISGWNGDNMI   200
EATTNAPWYK GWEKETKAGV VKGKTLLEAI DAIEQPSRPT DKPLRLPLQD   250
VYKIGGIGTV PVGRVETGVI KPGMVVTFAP AGVTTEVKSV EMHHEQLEQG   300
VPGDNVGFNV KNVSVKEIRR GNVCGDAKND PPKGCASFNA TVIVLNHPGQ   350
ISAGYSPVLD CHTAHIACRF DELLEKNDRR SGKKLEDHPK FLKSGDAALV   400
KFVPSKPMCV EAFSEYPPLG RFAVRDMRQT VAVGVIKSVD KTEKAAKVTK   450
AAQKAAKK                                                 458