SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTDH2
Systematic nameYJR009C
AliasGPD2
DescriptionGlyceraldehyde-3-phosphate dehydrogenase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP00358
SGD YJR009C
Gene Ontology (GO)
Cellular componentGO:0009277chitin- and beta-glucan-containing cell wallIDA:SGD.
Cellular componentGO:0005811lipid particleIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004365glyceraldehyde-3-phosphate dehydrogenase (p...IDA:SGD.
Biological processGO:0006094gluconeogenesisIEP:SGD.
Biological processGO:0006096glycolysisIEP:SGD.
EC number 1.2.1.12

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
49Phosphoserine.
50Phosphothreonine.
54Phosphotyrosine.
59Phosphoserine.
149Phosphoserine.
201Phosphoserine.
310Phosphoserine.
319Phosphoserine.
320Phosphothreonine.

Sequence Information
Sequence length332
Molecular weight35847

	---------+----------+----------+----------+----------+
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST   50
HGRYAGEVSH DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF   100
KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC   150
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA   200
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE   250
TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ   300
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA                      332