SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameTAT2
Systematic nameYOL020W
AliasLTG3, SAB2, SCM2, TAP2
DescriptionTryptophan permease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5 DOA4Vacuolar Degradation
2K10;K17;K20MULTI HECTNARSP5 DOA4Vacuolar Degradation
3       DOA4, UBP6, UBP14 
4   HECTNARSP5  Vacuolar Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Starvation induces vacuolar targeting and degradation of the tryptophan permease in yeast."
Beck T, Schmidt A, Hall MN.
J Cell Biol. 146(6):1227-38 (1999) [10491387]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane."
Umebayashi K, Nakano A.
J Cell Biol. 161(6):1117-31 (2003) [12810702]
[3]Evidence 
Method 
Reference"Multiple ubiquitin-specific protease genes are involved in degradation of yeast tryptophan permease Tat2 at high pressure."
Miura T, Abe F.
FEMS Microbiol Lett. 239(1):171-9 (2004) [15451116]
[4]EvidenceProtein Stability Assay
MethodWestern blot
Reference"The ergosterol biosynthesis inhibitor zaragozic acid promotes vacuolar degradation of the tryptophan permease Tat2p in yeast."
Daicho K, Maruyama H, Suzuki A, Ueno M, Uritani M, Ushimaru T.
Biochim Biophys Acta. 1768(7):1681-90 (2007) [17531951]

Cross References
Swiss-Prot accessionP38967
SGD YOL020W
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0015173aromatic amino acid transporter activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0015801aromatic amino acid transportIDA:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length592
Molecular weight65404

	---------+----------+----------+----------+----------+
MTEDFISSVK RSNEELKERK SNFGFVEYKS KQLTSSSSHN SNSSHHDDDN   50
QHGKRNIFQR CVDSFKSPLD GSFDTSNLKR TLKPRHLIMI AIGGSIGTGL   100
FVGSGKAIAE GGPLGVVIGW AIAGSQIIGT IHGLGEITVR FPVVGAFANY   150
GTRFLDPSIS FVVSTIYVLQ WFFVLPLEII AAAMTVQYWN SSIDPVIWVA   200
IFYAVIVSIN LFGVRGFGEA EFAFSTIKAI TVCGFIILCV VLICGGGPDH   250
EFIGAKYWHD PGCLANGFPG VLSVLVVASY SLGGIEMTCL ASGETDPKGL   300
PSAIKQVFWR ILFFFLISLT LVGFLVPYTN QNLLGGSSVD NSPFVIAIKL   350
HHIKALPSIV NAVILISVLS VGNSCIFASS RTLCSMAHQG LIPWWFGYID   400
RAGRPLVGIM ANSLFGLLAF LVKSGSMSEV FNWLMAIAGL ATCIVWLSIN   450
LSHIRFRLAM KAQGKSLDEL EFVSAVGIWG SAYSALINCL ILIAQFYCSL   500
WPIGGWTSGK ERAKIFFQNY LCALIMLFIF IVHKIYYKCQ TGKWWGVKAL   550
KDIDLETDRK DIDIEIVKQE IAEKKMYLDS RPWYVRQFHF WC           592

	* Ubiquitinated lysine is denoted as red color.