SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSWE1
Systematic nameYJL187C
DescriptionMitosis inhibitor protein kinase SWE1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTICDC34RINGSCFMET30   
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1."
Kaiser P, Sia RA, Bardes EG, Lew DJ, Reed SI.
Genes Dev. 12(16):2587-97 (1998) [9716410]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Determinants of Swe1p degradation in Saccharomyces cerevisiae."
McMillan JN, Theesfeld CL, Harrison JC, Bardes ES, Lew DJ.
Mol Biol Cell. 13(10):3560-75 (2002) [12388757]

Cross References
Swiss-Prot accessionP32944
SGD YJL187C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0000078cell morphogenesis checkpointIDA:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIDA:SGD.
Biological processGO:0008361regulation of cell sizeIMP:SGD.
Biological processGO:0000079regulation of cyclin-dependent protein kina...IDA:SGD.
Biological processGO:0040020regulation of meiosisIEP:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
262Phosphoserine.

Sequence Information
Sequence length819
Molecular weight92468

	---------+----------+----------+----------+----------+
MSSLDEDEED FEMLDTENLQ FMGKKMFGKQ AGEDESDDFA IGGSTPTNKL   50
KFYPYSNNKL TRSTGTLNLS LSNTALSEAN SKFLGKIEEE EEEEEEGKDE   100
ESVDSRIKRW SPFHENESVT TPITKRSAEK TNSPISLKQW NQRWFPKNDA   150
RTENTSSSSS YSVAKPNQSA FTSSGLVSKM SMDTSLYPAK LRIPETPVKK   200
SPLVEGRDHK HVHLSSSKNA SSSLSVSPLN FVEDNNLQED LLFSDSPSSK   250
ALPSIHVPTI DSSPLSEAKY HAHDRHNNQT NILSPTNSLV TNSSPQTLHS   300
NKFKKIKRAR NSVILKNREL TNSLQQFKDD LYGTDENFPP PIIISSHHST   350
RKNPQPYQFR GRYDNDTDEE ISTPTRRKSI IGATSQTHRE SRPLSLSSAI   400
VTNTTSAETH SISSTDSSPL NSKRRLISSN KLSANPDSHL FEKFTNVHSI   450
GKGQFSTVYQ VTFAQTNKKY AIKAIKPNKY NSLKRILLEI KILNEVTNQI   500
TMDQEGKEYI IDYISSWKFQ NSYYIMTELC ENGNLDGFLQ EQVIAKKKRL   550
EDWRIWKIIV ELSLALRFIH DSCHIVHLDL KPANVMITFE GNLKLGDFGM   600
ATHLPLEDKS FENEGDREYI APEIISDCTY DYKADIFSLG LMIVEIAANV   650
VLPDNGNAWH KLRSGDLSDA GRLSSTDIHS ESLFSDITKV DTNDLFDFER   700
DNISGNSNNA GTSTVHNNSN INNPNMNNGN DNNNVNTAAT KNRLILHKSS   750
KIPAWVPKFL IDGESLERIV RWMIEPNYER RPTANQILQT EECLYVEMTR   800
NAGAIIQEDD FGPKPKFFI                                     819