SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSUP1
Systematic nameYBR143C
AliasSAL4, SUP45
DescriptionEukaryotic peptide chain release factor subunit 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP12385
SGD YBR143C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIPI:SGD.
Cellular componentGO:0018444translation release factor complexIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0016149translation release factor activity, codon ...IMP:SGD.
Biological processGO:0000910cytokinesisIGI:SGD.
Biological processGO:0006415translational terminationIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
182N5-methylglutamine.

Sequence Information
Sequence length437
Molecular weight48991

	---------+----------+----------+----------+----------+
MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG LIPLYQKMLT   50
DEYGTASNIK SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE   100
DGKEKKVTFD IEPYKPINTS LYLCDNKFHT EVLSELLQAD DKFGFIVMDG   150
QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG GQSALRFARL REEKRHNYVR   200
KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD PRLACKVISI   250
VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY   300
GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS   350
FAIDKATGQE MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG   400
FGGIGAMLRY KVNFEQLVDE SEDEYYDEDE GSDYDFI                 437