SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSUB2
Systematic nameYDL084W
DescriptionATP-dependent RNA helicase SUB2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionQ07478
SGD YDL084W
Gene Ontology (GO)
Cellular componentGO:0000781chromosome, telomeric regionIPI:SGD.
Cellular componentGO:0005681spliceosomeTAS:SGD.
Cellular componentGO:0000346transcription export complexIPI:SGD.
Molecular functionGO:0004004ATP-dependent RNA helicase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIPI:SGD.
Biological processGO:0006348chromatin silencing at telomereIMP:SGD.
Biological processGO:0006406mRNA export from nucleusIDA:SGD.
Biological processGO:0000370U2-type nuclear mRNA branch site recognitionTAS:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
13Phosphoserine.
36Phosphothreonine.
55Phosphoserine.
61Phosphoserine.

Sequence Information
Sequence length446
Molecular weight50309

	---------+----------+----------+----------+----------+
MSHEGEEDLL EYSDNEQEIQ IDASKAAEAG ETGAATSATE GDNNNNTAAG   50
DKKGSYVGIH STGFKDFLLK PELSRAIIDC GFEHPSEVQQ HTIPQSIHGT   100
DVLCQAKSGL GKTAVFVLST LQQLDPVPGE VAVVVICNAR ELAYQIRNEY   150
LRFSKYMPDV KTAVFYGGTP ISKDAELLKN KDTAPHIVVA TPGRLKALVR   200
EKYIDLSHVK NFVIDECDKV LEELDMRRDV QEIFRATPRD KQVMMFSATL   250
SQEIRPICRR FLQNPLEIFV DDEAKLTLHG LQQYYIKLEE REKNRKLAQL   300
LDDLEFNQVI IFVKSTTRAN ELTKLLNASN FPAITVHGHM KQEERIARYK   350
AFKDFEKRIC VSTDVFGRGI DIERINLAIN YDLTNEADQY LHRVGRAGRF   400
GTKGLAISFV SSKEDEEVLA KIQERFDVKI AEFPEEGIDP STYLNN       446