SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTV1
Systematic nameYMR054W
DescriptionVacuolar ATP synthase subunit a, Golgi isoform

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP37296
SGD YMR054W
Gene Ontology (GO)
Cellular componentGO:0005794Golgi apparatusIDA:SGD.
Cellular componentGO:0005770late endosomeIDA:SGD.
Cellular componentGO:0000220vacuolar proton-transporting V-type ATPase,...IGI:SGD.
Molecular functionGO:0046961hydrogen ion transporting ATPase activity, ...IEP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007035vacuolar acidificationIGI:SGD.
EC number 3.6.3.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
223Phosphoserine.

Sequence Information
Sequence length890
Molecular weight101661

	---------+----------+----------+----------+----------+
MNQEEAIFRS ADMTYVQLYI PLEVIREVTF LLGKMSVFMV MDLNKDLTAF   50
QRGYVNQLRR FDEVERMVGF LNEVVEKHAA ETWKYILHID DEGNDIAQPD   100
MADLINTMEP LSLENVNDMV KEITDCESRA RQLDESLDSL RSKLNDLLEQ   150
RQVIFECSKF IEVNPGIAGR ATNPEIEQEE RDVDEFRMTP DDISETLSDA   200
FSFDDETPQD RGALGNDLTR NQSVEDLSFL EQGYQHRYMI TGSIRRTKVD   250
ILNRILWRLL RGNLIFQNFP IEEPLLEGKE KVEKDCFIIF THGETLLKKV   300
KRVIDSLNGK IVSLNTRSSE LVDTLNRQID DLQRILDTTE QTLHTELLVI   350
HDQLPVWSAM TKREKYVYTT LNKFQQESQG LIAEGWVPST ELIHLQDSLK   400
DYIETLGSEY STVFNVILTN KLPPTYHRTN KFTQAFQSIV DAYGIATYKE   450
INAGLATVVT FPFMFAIMFG DMGHGFILFL MALFLVLNER KFGAMHRDEI   500
FDMAFTGRYV LLLMGAFSVY TGLLYNDIFS KSMTIFKSGW QWPSTFRKGE   550
SIEAKKTGVY PFGLDFAWHG TDNGLLFSNS YKMKLSILMG YAHMTYSFMF   600
SYINYRAKNS KVDIIGNFIP GLVFMQSIFG YLSWAIVYKW SKDWIKDDKP   650
APGLLNMLIN MFLAPGTIDD QLYSGQAKLQ VVLLLAALVC VPWLLLYKPL   700
TLRRLNKNGG GGRPHGYQSV GNIEHEEQIA QQRHSAEGFQ GMIISDVASV   750
ADSINESVGG GEQGPFNFGD VMIHQVIHTI EFCLNCISHT ASYLRLWALS   800
LAHAQLSSVL WDMTISNAFS SKNSGSPLAV MKVVFLFAMW FVLTVCILVF   850
MEGTSAMLHA LRLHWVEAMS KFFEGEGYAY EPFSFRAIIE               890