SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTP2
Systematic nameYHR006W
DescriptionZinc finger protein STP2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38704
SGD YHR006W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIPI:SGD.
Molecular functionGO:0003704specific RNA polymerase II transcription fa...IGI:SGD.
Biological processGO:0045944positive regulation of transcription from R...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length541
Molecular weight60792

	---------+----------+----------+----------+----------+
MPILSLSSTR NSVLTRIYDY LKALVQQVIV PNVEDDKSSK STPFEKLEPA   50
KQNHPQKDCC ATEKDDLVDV SELFPKQNNK QLSLTSKSSV VPCALNLDNL   100
ETPFSIKIDN NGAVTTQLNL DEPILRGPSR GEPAKLQNDL ISSPPLEESY   150
INNDQYKALF PSNFLPITPV SSVITPASKK SIDESPLSDE VQGIADESSE   200
TLPYICHYCD ARFRIRGYLT RHIKKHAKRK AYHCPFFDNS ISQELRCHTS   250
GGFSRRDTYK THLKSRHFTY PEGVKPQDRN KSPGVCTQCG EHFSTSESWV   300
ENHIEAGSCK GLPEGYSEGI REKKKTSKMK MIKTSDGQTR FISSDESVSE   350
PALQNKNCIE ATVMQSKERP NDKIIPTKTE KNDFGIGTQW FERKQISRPT   400
QTTQSRGPTE VQNLKEWSII SPPILSPQNA SSVPQEYQSS RYTLHMDSPA   450
LSSASSALSP LSGDPITTTE TNKSYPLDSE QSLLEPDKTE EDAINQSKES   500
NMISINEMLQ KQMDFELLGE NHLKETQDYL ALYKKAYGIE F            541