SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTP1
Systematic nameYDR463W
AliasBAP1, SSY2
DescriptionZinc finger protein STP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ00947
SGD YDR463W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003704specific RNA polymerase II transcription fa...IGI:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Biological processGO:0045944positive regulation of transcription from R...IGI:SGD.
Biological processGO:0006388tRNA splicingIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
166Phosphoserine.
198Phosphoserine.

Sequence Information
Sequence length577
Molecular weight64868

	---------+----------+----------+----------+----------+
MNCITIDVFF TAFSHPCKRK KKQADSCINC KDSLIKSRFT LQTKGKGKLY   50
RLYHIRSKMP STTLLFPQKH IRAIPGKIYA FFRELVSGVI ISKPDLSHHY   100
SCENATKEEG KDAADEEKTT TSLFPESNNI DRSLNGGCSV IPCSMDVSDL   150
NTPISITLSP ENRIKSEVNA KSLLGSRPEQ DTGAPIKMST GVTSSPLSPS   200
GSTPEHSTKV LNNGEEEFIC HYCDATFRIR GYLTRHIKKH AIEKAYHCPF   250
FNSATPPDLR CHNSGGFSRR DTYKTHLKAR HVLYPKGVKP QDRNKSSGHC   300
AQCGEYFSTI ENFVENHIES GDCKALPQGY TKKNEKRSGK LRKIKTSNGH   350
SRFISTSQSV VEPKVLFNKD AVEAMTIVAN NSSGNDIISK YGNNKLMLNS   400
ENFKVDIPKR KRKYIKKKQQ QVSGSTVTTP EVATQNNQEV APDEISSATI   450
FSPFDTHLLE PVPSSSSESS AEVMFHGKQM KNFLIDINSF TNQQQQAQDN   500
PSFLPLDIEQ SSYDLSEDAM SYPIISTQSN RDCTQYDNTK ISQILQSQLN   550
PEYLSENHMR ETQQYLNFYN DNFGSQF                            577