SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSTI1
Systematic nameYOR027W
DescriptionHeat shock protein STI1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP15705
SGD YOR027W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIPI:SGD.
Molecular functionGO:0030189chaperone activator activityIDA:SGD.
Molecular functionGO:0030190chaperone inhibitor activityIDA:SGD.
Molecular functionGO:0030544Hsp70 protein bindingIDA:SGD.
Molecular functionGO:0051879Hsp90 protein bindingIDA:SGD.
Biological processGO:0006457protein foldingIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
227Phosphoserine.

Sequence Information
Sequence length589
Molecular weight66265

	---------+----------+----------+----------+----------+
MSLTADEYKQ QGNAAFTAKD YDKAIELFTK AIEVSETPNH VLYSNRSACY   50
TSLKKFSDAL NDANECVKIN PSWSKGYNRL GAAHLGLGDL DEAESNYKKA   100
LELDASNKAA KEGLDQVHRT QQARQAQPDL GLTQLFADPN LIENLKKNPK   150
TSEMMKDPQL VAKLIGYKQN PQAIGQDLFT DPRLMTIMAT LMGVDLNMDD   200
INQSNSMPKE PETSKSTEQK KDAEPQSDST TSKENSSKAP QKEESKESEP   250
MEVDEDDSKI EADKEKAEGN KFYKARQFDE AIEHYNKAWE LHKDITYLNN   300
RAAAEYEKGE YETAISTLND AVEQGREMRA DYKVISKSFA RIGNAYHKLG   350
DLKKTIEYYQ KSLTEHRTAD ILTKLRNAEK ELKKAEAEAY VNPEKAEEAR   400
LEGKEYFTKS DWPNAVKAYT EMIKRAPEDA RGYSNRAAAL AKLMSFPEAI   450
ADCNKAIEKD PNFVRAYIRK ATAQIAVKEY ASALETLDAA RTKDAEVNNG   500
SSAREIDQLY YKASQQRFQP GTSNETPEET YQRAMKDPEV AAIMQDPVMQ   550
SILQQAQQNP AALQEHMKNP EVFKKIQTLI AAGIIRTGR               589