SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTE6
Systematic nameYKL209C
DescriptionMating factor A secretion protein STE6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 UBC4, UBC5MULTI     Vacuolar Degradation
2 UBC4, UBC5     DOA4Proteasomal Degradation
3K1022        
4 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants."
Kolling R, Hollenberg CP.
EMBO J. 13(14):3261-71 (1994) [8045256]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae."
Loayza D, Michaelis S.
Mol Cell Biol. 18(2):779-89 (1998) [9447974]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP12866
SGD YKL209C
Gene Ontology (GO)
Cellular componentGO:0005887integral to plasma membraneTAS:SGD.
Cellular componentGO:0043332mating projection tipIDA:SGD.
Molecular functionGO:0042626ATPase activity, coupled to transmembrane m...IDA:SGD.
Biological processGO:0000770peptide pheromone exportTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1290
Molecular weight144766

	---------+----------+----------+----------+----------+
MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR   50
VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG   100
ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE   150
ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV   200
YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF   250
IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC   300
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL   350
DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST   400
LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR   450
KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG   500
TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH   550
WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST   600
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE   650
TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI   700
IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD   750
GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM   800
EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS   850
TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL   900
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS   950
MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS   1000
QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP   1050
IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL   1100
TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY   1150
GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI   1200
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM   1250
MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS               1290

	* Ubiquitinated lysine is denoted as red color.