SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTE3
Systematic nameYKL178C
DescriptionPheromone a factor receptor

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 PLURIUBC4, UBC5      
2K432;K424;K453PLURI, MULTI      Vacuolar Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Ubiquitination of the yeast a-factor receptor."
Roth AF, Davis NG.
J Cell Biol. 134(3):661-74 (1996) [8707846]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Ubiquitination of the PEST-like endocytosis signal of the yeast a-factor receptor."
Roth AF, Davis NG.
J Biol Chem. 275(11):8143-53 (2000) [10713137]

Cross References
Swiss-Prot accessionP06783
SGD YKL178C
Gene Ontology (GO)
Cellular componentGO:0005887integral to plasma membraneIDA:SGD.
Molecular functionGO:0004933mating-type a-factor pheromone receptor act...IMP:SGD.
Biological processGO:0000750pheromone-dependent signal transduction dur...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length470
Molecular weight53708

	---------+----------+----------+----------+----------+
MSYKSAIIGL CLLAVILLAP PLAWHSHTKN IPAIILITWL LTMNLTCIVD   50
AAIWSDDDFL TRWDGKGWCD IVIKLQVGAN IGISCAVTNI IYNLHTILKA   100
DSVLPDLSSW TKIVKDLVIS LFTPVMVMGF SYLLQVFRYG IARYNGCQNL   150
LSPTWITTVL YTMWMLIWSF VGAVYATLVL FVFYKKRKDV RDILHCTNSG   200
LNLTRFARLL IFCFIIILVM FPFSVYTFVQ DLQQVEGHYT FKNTHSSTIW   250
NTIIKFDPGR PIYNIWLYVL MSYLVFLIFG LGSDALHMYS KFLRSIKLGF   300
VLDMWKRFID KNKEKRVGIL LNKLSSRKES RNPFSTDSEN YISTCTENYS   350
PCVGTPISQA HFYVDYRIPD DPRKSQNKSK KYLFADKETD DILDEIDLKE   400
SRHIPYVTQG QSFDDEISLG GFSKVTLDYS EKLHNSASSN FEGESLCYSP   450
ASKEENSSSN EHSSENTAGP                                     470

	* Ubiquitinated lysine is denoted as red color.