SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTE2
Systematic nameYFL026W
DescriptionPheromone alpha factor receptor

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K337;374MONO       
2K337MONO       
3 MONOUBC1, UBC4, UBC5HECTNARSP5  Vacuolar Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
Methodautoradiography
Reference"Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis."
Hicke L, Riezman H.
Cell. 84(2):277-87 (1996) [8565073]
[2]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"A function for monoubiquitination in the internalization of a G protein-coupled receptor."
Terrell J, Shih S, Dunn R, Hicke L.
Mol Cell. 1(2):193-202 (1998) [9659916]
[3]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Domains of the Rsp5 ubiquitin-protein ligase required for receptor-mediated and fluid-phase endocytosis."
Dunn R, Hicke L.
Mol Biol Cell. 12(2):421-35 (2001) [11179425]

Cross References
Swiss-Prot accessionP06842
SGD YFL026W
Gene Ontology (GO)
Cellular componentGO:0005887integral to plasma membraneIDA:SGD.
Molecular functionGO:0004934mating-type alpha-factor pheromone receptor...IDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0030031cell projection biogenesisIMP:SGD.
Biological processGO:0000749response to pheromone during conjugation wi...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
310Phosphoserine.
329Phosphothreonine.
331Phosphoserine.
360Phosphoserine.
363Phosphothreonine.
366Phosphoserine.
382Phosphothreonine.
385Phosphoserine.
386Phosphoserine.
411Phosphothreonine.
414Phosphothreonine.

Sequence Information
Sequence length431
Molecular weight47849

	---------+----------+----------+----------+----------+
MSDAAPSLSN LFYDPTYNPG QSTINYTSIY GNGSTITFDE LQGLVNSTVT   50
QAIMFGVRCG AAALTLIVMW MTSRSRKTPI FIINQVSLFL IILHSALYFK   100
YLLSNYSSVT YALTGFPQFI SRGDVHVYGA TNIIQVLLVA SIETSLVFQI   150
KVIFTGDNFK RIGLMLTSIS FTLGIATVTM YFVSAVKGMI VTYNDVSATQ   200
DKYFNASTIL LASSINFMSF VLVVKLILAI RSRRFLGLKQ FDSFHILLIM   250
SCQSLLVPSI IFILAYSLKP NQGTDVLTTV ATLLAVLSLP LSSMWATAAN   300
NASKTNTITS DFTTSTDRFY PGTLSSFQTD SINNDAKSSL RSRLYDLYPR   350
RKETTSDKHS ERTFVSETAD DIEKNQFYQL PTPTSSKNTR IGPFADASYK   400
EGEVEPVDMY TPDTAADEEA RKFWTEDNNN L                       431

	* Ubiquitinated lysine is denoted as red color.