SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSTD1
Systematic nameYOR047C
AliasMSN3, SFS3
DescriptionProtein STD1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGSCFGRR1   
2K207;K287;K312;K337;K344;K347;K354;K381;K411 CDC34RINGSCFGRR1  Proteasomal Degradation
3   RINGSCFGRR1   
4        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters."
Flick KM, Spielewoy N, Kalashnikova TI, Guaderrama M, Zhu Q, Chang HC, Wittenberg C.
Mol Biol Cell. 14(8):3230-41 (2003) [12925759]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Integration of transcriptional and posttranslational regulation in a glucose signal transduction pathway in Saccharomyces cerevisiae."
Kim JH, Brachet V, Moriya H, Johnston M.
Eukaryot Cell. 5(1):167-73 (2006) [16400179]
[3]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Biochemical evidence for glucose-independent induction of HXT expression in Saccharomyces cerevisiae."
Pasula S, Jouandot D 2nd, Kim JH.
FEBS Lett. 581(17):3230-4 (2007) [17586499]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ02794
SGD YOR047C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0030295protein kinase activator activityIGI:SGD.
Biological processGO:0006006glucose metabolic processIMP:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IMP:SGD.
Biological processGO:0009651response to salt stressIMP:SGD.
Biological processGO:0007165signal transductionIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length444
Molecular weight50261

	---------+----------+----------+----------+----------+
MFVSPPPATA RNQVLGKRKS KRHDENPKNV QPNADTEMTN SVPSIGFNSN   50
LPHNNQEINT PNHYNLSSNS GNVRSNNNFV TTPPEYADRA RIEIIKRLLP   100
TAGTKPMEVN SNTAENANIQ HINTPDSQSF VSDHSSSYES SIFSQPSTAL   150
TDITTGSSLI DTKTPKFVTE VTLEDALPKT FYDMYSPEVL MSDPANILYN   200
GRPKFTKREL LDWDLNDIRS LLIVEQLRPE WGSQLPTVVT SGINLPQFRL   250
QLLPLSSSDE FIIATLVNSD LYIEANLDRN FKLTSAKYTV ASARKRHEEM   300
TGSKEPIMRL SKPEWRNIIE NYLLNVAVEA QCRYDFKQKR SEYKRWKLLN   350
SNLKRPDMPP PSLIPHGFKI HDCTNSGSLL KKALMKNLQL KNYKNDAKTL   400
GAGTQKNVVN KVSLTSEERA AIWFQCQTQV YQRLGLDWKP DGMS         444

	* Ubiquitinated lysine is denoted as red color.