SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSSK1
Systematic nameYLR006C
DescriptionOsomolarity two-component system protein SSK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ07084
SGD YLR006C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmTAS:SGD.
Molecular functionGO:0008047enzyme activator activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0000156two-component response regulator activityTAS:SGD.
Biological processGO:0007234osmosensory signaling pathway via two-compo...IDA:SGD.
Biological processGO:0042542response to hydrogen peroxideIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
110Phosphoserine.
186Phosphoserine.
351Phosphoserine.
368Phosphoserine.
380Phosphoserine.
5544-aspartylphosphate.
673Phosphoserine.
693Phosphothreonine.
695Phosphothreonine.
697Phosphoserine.
706Phosphoserine.

Sequence Information
Sequence length712
Molecular weight78529

	---------+----------+----------+----------+----------+
MLNSALLWKV WLRIDNSTDE VNQPIAVQFD EIDTVDDLKS RFFQKLSSTR   50
WREINDNASI AIGLYAPKFD NQADNTSSNN TNDNSCRSKS NGAGSGANLS   100
VNSNTKSSVS PTAGSFGLSK DLAKDRNVLQ HPKPTQKRGA LYDAFAAVPT   150
VAATTNVDFP PNEAPMLSPQ RPYSTSPKQF PATTKSPLLR FASVSPYPKF   200
HSDNQIMASA GLTYVSPHNK NKYTRPLIRK GLNFTTESVN DCTYKIIFEP   250
DELAINIYKE LFGTMGSQPA SQPLLIFSNV NLRQDVPPLD ILNVVDYVPT   300
NEEISQQKTQ PTDHGAVGVF HLDDHISPGE QGLKQTIGDK ADLKGKDGNS   350
SPQEFKLITD EEQLRRASQE LKDEEKDAES PWQAILLLPK GYKGGVDFRN   400
KPVAHTDSSF NNEDTITHSE LEVNTGSPSQ ESGSLNEAGI GITQPMSEVQ   450
RRKEDVTPAS PILTSSQTPH YSNSLYNAPF AVSSPPDPLP NLFTTTSEKV   500
FPKINVLIVE DNVINQAILG SFLRKHKISY KLAKNGQEAV NIWKEGGLHL   550
IFMDLQLPVL SGIEAAKQIR DFEKQNGIGI QKSLNNSHSN LEKGTSKRFS   600
QAPVIIVALT ASNSQMDKRK ALLSGCNDYL TKPVNLHWLS KKITEWGCMQ   650
ALIDFDSWKQ GESRMTDSVL VKSPQKPIAP SNPHSFKQAT SMTPTHSPVR   700
KNSNLSPTQI EL                                            712