SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSSH4
Systematic nameYKL124W
AliasMLF4
DescriptionProtein SSH4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32343
SGD YKL124W
Gene Ontology (GO)
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0016192vesicle-mediated transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
358Phosphoserine.

Sequence Information
Sequence length579
Molecular weight65061

	---------+----------+----------+----------+----------+
MYVTFNEALD SSFGNLESPN HDFKVGDPNM VPTPPMDSDS AAISLAFLIS   50
LSITFAILML ILVVIAAYVT FCGDDESEYD EENALGTRTS GTLHSLFGKK   100
HSGILLDSSF ASPGGFDDEI VLQERELEEL PKMSAYEVEL YIRAKEFQMM   150
SPPMVKDFGT YLDSDDQQFI KDRGIQSYFL LPSINDNIDE YGNFLPSFIV   200
QDKLDIQFSK FNKSSSTVMN YPLPHNRKDA VYFEVKIFRH IQKSNSIFSI   250
GLTTVPYPYF RVPGMAKYSI AYESTGKLRI NNPFTASTLL PKLEEGDTVG   300
FGYRYKTGTI FITHNGKKLM DVTQNIGIDL FIGIGAFNAA YTRTYTRDGL   350
LEDPDNVSFR EALSEGKDIE VAKDLQRVHD PHDESDEMTS DEVELHVNLG   400
QVGFVFIEAN VKKYAFGSVY GQIGIPPAYN GTEIKKDTIL QKGEELPPRY   450
ADTDNFFGSM KVKEGSSSRI TAQTSKPLWS VGTYERISSN FDRENNVYHD   500
SLETDDNNTD NNVNNNDENA GCNENSPLLE DDGNKRPENS NTPREVSDGA   550
INKNPRNKST KKRQRNRGKS SKKKNRSRK                          579