SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSSF2
Systematic nameYDR312W
DescriptionRibosome biogenesis protein SSF2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ12153
SGD YDR312W
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Molecular functionGO:0019843rRNA bindingIPI:SGD.
Biological processGO:0000747conjugation with cellular fusionIGI:SGD.
Biological processGO:0000027ribosomal large subunit assembly and mainte...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
258Phosphoserine.

Sequence Information
Sequence length453
Molecular weight51856

	---------+----------+----------+----------+----------+
MAKRRQKKRT HAQITPEQER DIPKSMVIRV GQTSLANHSL NQLVKDFRQI   50
MQPHTAVKLK ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR   100
TPQGPTVTFQ VLDYSLGRDI KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK   150
RSDEDDQDVN VEKVIVSMFQ NIFPPLNPAR TSLNSIKRIF MINKDRETGE   200
ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE DISSLILDHD   250
LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ TSLSQKTPVK MTDNEEREKG   300
IEEEDVEMEE PKPSENSQPT PRKKAIKLTE LGPRLTLKLV KIEDGICSGK   350
VLHHEFVQKS SEEIKALEKR HAAKMRLKEQ RRKEQEENIA KKKAVKDAKK   400
QRKLERRKAR AEEQGEGQGK DGAMSDDGSS SSEDEHYSDV PEDLDSDLFS   450
EVE                                                      453