SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSSF1
Systematic nameYHR066W
DescriptionRibosome biogenesis protein SSF1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP38789
SGD YHR066W
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Molecular functionGO:0019843rRNA bindingIPI:SGD.
Biological processGO:0000747conjugation with cellular fusionIGI:SGD.
Biological processGO:0008361regulation of cell sizeIMP:SGD.
Biological processGO:0000027ribosomal large subunit assembly and mainte...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
301Phosphothreonine.

Sequence Information
Sequence length453
Molecular weight51764

	---------+----------+----------+----------+----------+
MAKRRQKKRT HAQLTPEQEQ GIPKSMVIRV GQTSLANHSL NQLVKDFRQI   50
MQPHTAIKLK ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR   100
TPQGPTVTFQ VLDYSLGRDI KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK   150
RSGEDDQDVN VEKVIVSMFQ NIFPPLNPAR TSLNSIKRVF MINKDRETGE   200
ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE DISSLILDHD   250
LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ SLKSQRTPVE KKDNKEREKE   300
TEEEDVEMEE PKPSENLQPT PRKKAIKLTE LGPRLTLKLV KIEEGICSGK   350
VLHHEFVQKS SEEIKALEKR HAAKMRLKEQ RKKEQEENIA KKKAVKDAKK   400
QRKLERRKAR AAEGGEGQGK DDAMSDDESS SSDSEHYGSV PEDLDSDLFS   450
EVE                                                      453