SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSSA3
Systematic nameYBL075C
DescriptionHeat shock protein SSA3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP09435
SGD YBL075C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Molecular functionGO:0016887ATPase activityIGI:SGD.
Molecular functionGO:0051082unfolded protein bindingIGI:SGD.
Biological processGO:0006457protein foldingIGI:SGD.
Biological processGO:0006950response to stressIMP:SGD.
Biological processGO:0006616SRP-dependent cotranslational protein targe...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length649
Molecular weight70547

	---------+----------+----------+----------+----------+
MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG   50
DAAKNQAAIN PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV   100
VQVEYKGETK TFTPEEISSM VLSKMKETAE NYLGTTVNDA VVTVPAYFND   150
SQRQATKDAG TIAGMNVLRI INEPTAAAIA YGLDKKGRAE HNVLIFDLGG   200
GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA TEFKRKTKKD   250
ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF   300
EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD   350
FFNGKEPNRS INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG   400
IETAGGIMTK LIPRNSTIPT KKSETFSTYA DNQPGVLIQV FEGERTRTKD   450
NNLLGKFELS GIPPAPRGVP QIDVTFDIDA NGILNVSALE KGTGKSNKIT   500
ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL ESYAFTLKNT   550
INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI   600
ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD    649