SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSPT23
Systematic nameYKL020C
DescriptionProtein SPT23

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   HECTNARSP5   
2         
3       OTU1 
4 MULTI HECTNARSP5CDC48-UFD1/NPL4, RAD23, DSK2, RPN10 Proteasomal Degradation
5        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidenceHigh MW Product Confirmation
Methodautoradiography (in vitro)
Reference"A single PXY motif located within the carboxyl terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p."
Shcherbik N, Kee Y, Lyon N, Huibregtse JM, Haines DS.
J Biol Chem. 279(51):53892-8 (2004) [15466864]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone."
Rumpf S, Jentsch S.
Mol Cell. 21(2):261-9 (2006) [16427015]
[4]EvidenceProtein Stability Assay
MethodWestern blot
Reference"A series of ubiquitin binding factors connects CDC48/p97 to substrate multiubiquitylation and proteasomal targeting."
Richly H, Rape M, Braun S, Rumpf S, Hoege C, Jentsch S.
Cell. 120(1):73-84 (2005) [15652483]
[5]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP35210
SGD YKL020C
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneIDA:SGD.
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0003677DNA bindingIPI:SGD.
Molecular functionGO:0016563transcriptional activator activityIMP:SGD.
Biological processGO:0006631fatty acid metabolic processIMP:SGD.
Biological processGO:0045944positive regulation of transcription from R...IMP:SGD.
Biological processGO:0009409response to coldIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
595Phosphoserine.
827Phosphoserine.

Sequence Information
Sequence length1082
Molecular weight121338

	---------+----------+----------+----------+----------+
MMSGTGNVSS MLHSYSANIQ HNDGSPDLDL LESELLDIAL LNSGSSLQDP   50
GLLSLNQEKM ITAGTTTPGK EDEGELRDDI ASLQGLLDRH VQFGRKLPLR   100
TPYANPLDFI NINPQSLPLS LEIIGLPKVS RVETQMKLSF RIRNAHARKN   150
FFIHLPSDCI AKDKFFTSSD DPTNLTIPNR DINERTLFLD AFLLCASNNN   200
SNNFKQTYVC NRCINREKRR ASRRKSGLND NSIWQNNENK RAIIFNSKQL   250
FIISNNGLSG NSNCINFDLP TRIVCYCRHH KATNGFVVLF LLRDHNGDIL   300
AKTITDPIMI MDKKNASNTT TPTSTSNAQV SPMTNDTRSF SSPQSDLNFP   350
SEFPLPSNSK NFVISTNCML DSNCNNNNND NDNKNNIKTN TAMMNNNRHF   400
PSPNSSSEDS NHSFSDIHFS NNNDNNLHRS LDSWSSTGFN SSSNPALTTL   450
TSDFSAASAR HTGKRQRSVN EPFMSTPNTF SRLPQKFIDS SKDISNHNSV   500
PVALNNKPSI QRVIPAQGSI NGGIEVTLLG SKFKQGLIIK FGENIALSSQ   550
CWNESTMVTY LPPSSKPGPV LVTIVDPSET SMRNNSNSSV STSNSTNDIL   600
HLNKYTGEKA IFTYVDDTDR QLIELALQIV GLKMNGKLED ARNIAKRIVG   650
SDSSPSNNNA GLHSQNSSLN SYTNMMRNIN DEQLITEVIK SFKRNNNLST   700
VNLSMCDVRG RTLLHLAAFN NWYSLVSLLI KYGSHLNDQD LFGFTPLHMA   750
CINGDLRIIR LLLECNVNIM KKTRNGFIAK QFFLMNYTVN KTRYSNYETS   800
LFDDILTRLT KNTTGSSDTQ PFERNVSQSS FNSSLFDDDD ADHDYVQERK   850
YLLADSAALA PEQSNCNDNT SFSILDSDSG YDISDCESSS DEIALEFFNT   900
HKIKDFSSKP NEIPKTTKTS IEPDGSLWNR MLTRLNDELP KYEDLFPKKP   950
KNWELGSKSV EIGPDNSAQM TVDDSQTSSE DDELEALQVG FNTIFSKKQN   1000
FQNDKMLLFF WIPLTLVLLL CFTLSNLGKD DDMFHNLSKI VQEYLRIGLA   1050
KVLLGNERMK TSFKMQLSKF QNNNILNDMR VN                      1082