SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSPT14
Systematic nameYPL175W
AliasCWH6, GPI3
DescriptionPhosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32363
SGD YPL175W
Gene Ontology (GO)
Cellular componentGO:0000506glycosylphosphatidylinositol-N-acetylglucos...TAS:SGD.
Molecular functionGO:0008194UDP-glycosyltransferase activityIPI:SGD.
Biological processGO:0006506GPI anchor biosynthetic processTAS:SGD.
EC number 2.4.1.198

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length461
Molecular weight52372

	---------+----------+----------+----------+----------+
MSSSHKVESY NNENRMLCDF FYPQLGGVEF HIYHLSQKLI DLGHSVVIIT   50
HAYKDRVGVR HLTNGLKVYH VPFFVIFRET TFPTVFSTFP IIRNILLREQ   100
IQIVHSHGSA STFAHEGILH ANTMGLRTVF TDHSLYGFNN LTSIWVNKLL   150
TFTLTNIDRV ICVSNTCKEN MIVRTELSPD IISVIPNAVV SEDFKPRDPT   200
GGTKRKQSRD KIVIVVIGRL FPNKGSDLLT RIIPKVCSSH EDVEFIVAGD   250
GPKFIDFQQM IESHRLQKRV QLLGSVPHEK VRDVLCQGDI YLHASLTEAF   300
GTILVEAASC NLLIVTTQVG GIPEVLPNEM TVYAEQTSVS DLVQATNKAI   350
NIIRSKALDT SSFHDSVSKM YDWMDVAKRT VEIYTNISST SSADDKDWMK   400
MVANLYKRDG IWAKHLYLLC GIVEYMLFFL LEWLYPRDEI DLAPKWPKKT   450
VSNETKEARE T                                             461