SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSPS1
Systematic nameYDR523C
DescriptionSporulation-specific protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP08458
SGD YDR523C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0005628prospore membraneIDA:SGD.
Biological processGO:0030476spore wall assembly (sensu Fungi)IMP:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length490
Molecular weight55704

	---------+----------+----------+----------+----------+
MESKEISIRS RTPPSKLYSI QSCIGRGNFG DVYKAVDRVT QEIVAIKVVN   50
LEHSDEDIEL LAQEIFFLAE LKSPLITNYI ATMLEDVSMW IVMEYCGGGS   100
CSDLLKRSYV NGLPEEKVSF IIHEVTLGLK YLHEQRKIHR DIKAANILLN   150
EEGMVKLGDF GVSGHIRSTL KRDTFVGTPY WMAPEVVCCE VDGYNEKADI   200
WSLGITTYEL LKGLPPLSKY DPMKVMTNLP KRKPPKLQGP FSDAAKDFVA   250
GCLVKTPADR PSAYNLLSFE FVKNITITNL KSDVDLIKQK KVQERYTKVP   300
KYPLQNRLYK NSNTVRGKEF WNFESTRLST TQISKEELSP ITQDSPTSSL   350
NMESPYLLHG QTVTPITNPS SSSFRKCTQP VFELDSGMDI DSGCPNAQAE   400
TEIVPLSNHN KKHKKNDIQA LKIEKFDYLK NIVSHILNRM YDRARDDETR   450
KYVNEMLKQF IKTEANVPGF NEVFIEEISL RIEAIKKGFV               490