SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSPP41
Systematic nameYDR464W
DescriptionProtein SPP41

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38904
SGD YDR464W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Biological processGO:0000122negative regulation of transcription from R...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
519Phosphoserine.
563Phosphoserine.
1014Phosphoserine.
1067Phosphoserine.

Sequence Information
Sequence length1435
Molecular weight161597

	---------+----------+----------+----------+----------+
MAYDEDDGEI NFNELVGNLL SSHNQEGQEE GEVQGGEQEG DDFEKIYPTS   50
ENIEPKHPDD SQHMHNSPDQ NIEIPHFVDE EDELVSVVAN AVQNIDDEQA   100
KPENHLENGS EHVTSDTADD NHEKEQQQEW AHILQQEILK SDGEPLRENT   150
ERRVSTSQHH PSQRTDDALD QDDENLRMAI LESLQELNTN EEEEKEPEKH   200
EHAAPNDKLS SKKSSKKKKK DKSKNRESSK DKSSKKSKSS SHSKKHAKDR   250
NKEKQSKPTN NENTLDLSNI LENLIHENDN AAIDTAKQTV DIQDNSHTDN   300
TNNEDVEAQA LVEATLKAFE NELLSSAPTE EPSQEQSIGP VSSRKAVEPP   350
RKPTADDIPL AMLQAFKPKK RPPQEKKKTK SKTSKAASTA NKSPASESTS   400
KKKKKKKTVK ESNKSQEAYE DDEFSRILAD MVNQVVNTSL KETSTHTATQ   450
DNKLESESDF TSPVQSQYTT EDASTANDDS LDLNQIMQNA MAMVFQNQND   500
DEFDENIVED FNRGLGDLSV SDLLPHDNLS RMEKKSVPKS SSKSEKKTAI   550
SRRASKKASR DASSVELTEV PSKPKKPSKT EVSLEKKLRK KYVSIANEAA   600
SVARKKRWAK NKELKEKEKL ERQTAREERR HKKKLEKQRL AEEQEELKKI   650
VERGPPYPPD LRLTKSGKPK KPYRRWTPEE LLKRSQEAEK PRKVKKERKK   700
KEKKMKVPSS ALKKIPLFNF VKGNVQPSAR HRLNDIEGSL STIGLHKSPD   750
GVRRILSRPK SEDHEWPLSD SSASQNYDAH LKTVVHKEKI PFHPPWTIPS   800
QPPFALPVAR RKKIPNIKKY RKRTNNSFRV SKEGTASTRN RILPAILLPI   850
INTLKAAAKS QTAAGATPEE ARKRLATIIQ HAKSTVIRAA LQARKNSMQA   900
AHSKGTTTEL ATTASRMKNP LKMIPIFNTS RVKQQLDKQL PARSAGTEIS   950
SSESPDKATP DPHSNSTIAG HTLKGVTTPI KIEDSDANVP PVSIAVSTIE   1000
PSQDKLELTK RAESVEPVEN NVETAKETQS VQEIKENVGT KASEEVTLTE   1050
DKTNGDPKNE KRILIESPVE KTDKKKPGEK IATDLNEDAS LSDKKDGDEK   1100
STLHSDAAQL TGNEPDSVNT TTGKPKLIDV SLKPLNEAKP KIPIIFPLKR   1150
PQIKPEVSVI NLVQNLVNTK IPEIKNESVD LGSNITDILS STITNILPEI   1200
TATDVKNYQY EDENVKYLKK TPRQVLNLDG LVPPSGRCIT KAKRVRRIKK   1250
LSADATTAPE ADGKANSESI TYTFDIPSPE EVQSKRSVVL KFAKARLTEA   1300
ELSCLKKEIN NVRKRRWREM NSTKNWEYDV KSRLKKRANA FFGEGESETK   1350
SKWIEERFQE KVSQEKYKDR LETTETQANN TKIVIDDKEI LNILAVNMNN   1400
LNKARCIEKD IQESFREEKL ASLQPKKKRK KSILH                   1435