SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSPC42
Systematic nameYKL042W
DescriptionSpindle pole body component SPC42

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36094
SGD YKL042W
Gene Ontology (GO)
Cellular componentGO:0005823central plaque of spindle pole bodyIDA:SGD.
Cellular componentGO:0005821intermediate layer of spindle pole bodyIDA:SGD.
Molecular functionGO:0005200structural constituent of cytoskeletonIPI:SGD.
Biological processGO:0007020microtubule nucleationIPI:SGD.
Biological processGO:0007103spindle pole body duplication in nuclear en...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
12Phosphoserine.
213Phosphoserine.
217Phosphoserine.

Sequence Information
Sequence length363
Molecular weight42271

	---------+----------+----------+----------+----------+
MNGSPTPKRY SSKSSRLYDD YYNIPYQYSN PTPMNRDYND VGSRINADKL   50
VPEEYKRNTE FINKAVQQNK ELNFKLREKQ NEIFELKKIA ETLRSKLEKY   100
VDITKKLEDQ NLNLQIKISD LEKKLSDANS TFKEMRFPKV KDPMVDDDPV   150
SENYDQINVP KHRAPDATGN PRTTNKVSNT SDQDSRLKAI ERTLSVLTNY   200
VMRSEDGNND RMSPLPSPLN TILPINNRLN FQEPKRYNPT VKVNPSDDDI   250
MMYESAELKR VEEEIEELKR KILVRKKHDL RKLSLNNQLQ ELQSMMDGDD   300
NIKLDNVSKH NHATHRHSSQ SSRDYSPSSD ACLECSNDLY EKNRVKPENN   350
MSETFATPTP NNR                                           363