SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSNF1
Systematic nameYDR477W
AliasCAT1, CCR1, GLC2, PAS14
DescriptionCarbon catabolite-derepressing protein kinase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP06782
SGD YDR477W
Gene Ontology (GO)
Cellular componentGO:0031588AMP-activated protein kinase complexIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005641nuclear envelope lumenIDA:SGD.
Cellular componentGO:0000324vacuole, cell cycle-correlated morphologyIPI:SGD.
Molecular functionGO:0004679AMP-activated protein kinase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0042710biofilm formationIMP:SGD.
Biological processGO:0007155cell adhesionIMP:SGD.
Biological processGO:0006995cellular response to nitrogen starvationIDA:SGD.
Biological processGO:0001403invasive growth (sensu Saccharomyces)IMP:SGD.
Biological processGO:0045722positive regulation of gluconeogenesisIMP:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
Biological processGO:0001302replicative cell agingIGI:SGD.
Biological processGO:0007165signal transductionTAS:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
210Phosphothreonine, by autocatalysis.
413Phosphoserine.

Sequence Information
Sequence length633
Molecular weight72045

	---------+----------+----------+----------+----------+
MSSNNNTNTA PANANSSHHH HHHHHHHHHH GHGGSNSTLN NPKSSLADGA   50
HIGNYQIVKT LGEGSFGKVK LAYHTTTGQK VALKIINKKV LAKSDMQGRI   100
EREISYLRLL RHPHIIKLYD VIKSKDEIIM VIEYAGNELF DYIVQRDKMS   150
EQEARRFFQQ IISAVEYCHR HKIVHRDLKP ENLLLDEHLN VKIADFGLSN   200
IMTDGNFLKT SCGSPNYAAP EVISGKLYAG PEVDVWSCGV ILYVMLCRRL   250
PFDDESIPVL FKNISNGVYT LPKFLSPGAA GLIKRMLIVN PLNRISIHEI   300
MQDDWFKVDL PEYLLPPDLK PHPEEENENN DSKKDGSSPD NDEIDDNLVN   350
ILSSTMGYEK DEIYESLESS EDTPAFNEIR DAYMLIKENK SLIKDMKANK   400
SVSDELDTFL SQSPPTFQQQ SKSHQKSQVD HETAKQHARR MASAITQQRT   450
YHQSPFMDQY KEEDSTVSIL PTSLPQIHRA NMLAQGSPAA SKISPLVTKK   500
SKTRWHFGIR SRSYPLDVMG EIYIALKNLG AEWAKPSEED LWTIKLRWKY   550
DIGNKTNTNE KIPDLMKMVI QLFQIETNNY LVDFKFDGWE SSYGDDTTVS   600
NISEDEMSTF SAYPFLHLTT KLIMELAVNS QSN                     633